Associate Professor Steve Johnston

Featured projects	Duration
Innovative reproductive techniques for preserving marsupial species

Gosálvez, Jaime, Vargas-Baquero, Eduardo and Johnston, Stephen D. (2022). Sperm DNA fragmentation and its relevance to men with spinal cord injury. Cellular, molecular, physiological, and behavioral aspects of spinal cord injury: The Neuroscience of Spinal Cord Injury. (pp. 93-104) edited by Rajkumar Rajendram, Victor R. Preedy and Colin R. Martin. London, United Kingdom: Elsevier. doi: 10.1016/b978-0-12-822427-4.00008-3

Keeley, Tamara and Johnston, Stephen (2019). Assessment and management of reproduction in Australian monotremes and marsupials. Current therapy in medicine of Australian mammals. (pp. 63-84) edited by Larry Vogelnest and Timothy Portas. Clayton South, VIC, Australia: CSIRO Publishing.

Johnston, Stephen D. and Holt, William V. (2019). Using the koala (phascolarctos cinereus) as a case study to illustrate the development of artificial breeding technology in marsupials: An update. Reproductive sciences in animal conservation. (pp. 327-362) Cham, Switzerland: Springer Nature Switzerland. doi: 10.1007/978-3-030-23633-5_12

Fernandez, Jose Luis, Johnston, Stephen and Gosalvez, Jaime (2018). Sperm chromatin dispersion (SCD) assay. A clinician’s guide to sperm DNA and chromatin damage. (pp. 137-152) edited by Armand Zini and Ashok Agarwal. Cham, Switzerland: Spinger. doi: 10.1007/978-3-319-71815-6_8

Vincent Holt, William and Johnston, Stephen (2018). Mammalian reproduction overview. Encyclopedia of reproduction. (pp. 486-494) Cambridge, MA United States: Academic Press. doi: 10.1016/b978-0-12-809633-8.20508-6

Johnston, Stephen and Keeley, Tamara (2015). Enigmas in reproductive biology. Marsupials and monotremes: nature's enigmatic mammals. (pp. 145-227) edited by Athol Klieve, Lindsay Hogan, Stephen Johnston and Peter Murray. New York, NY, United States: Nova Science Publishers.

Gosalvez, Jaime, Holt, William V. and Johnston, Stephen D. (2014). Sperm DNA Fragmentation and its role in wildlife conservation. Reproductive sciences in animal conservation: Progress and prospects. (pp. 357-384) edited by William V. Holt, Janine L. Brown and Pierre Comizzoli. New York, NY United States: Springer. doi: 10.1007/978-1-4939-0820-2-15

Johnston, Stephen D. and Holt, William V. (2014). The koala (Phascolarctos cinereus): A case study in the development of reproductive technology in a marsupial. Reproductive sciences in animal conservation: Progress and prospects. (pp. 171-203) edited by William V. Holt, Janine L. Brown and Pierre Comizzoli. New York NY United States: Springer. doi: 10.1007/978-1-4939-0820-2-9

Johnston, Stephen (2013). Workshop Session3: Captive management in global koala conservation (local, national and international refuges for koala genotypes). Conserving central Queensland's koalas. (pp. 60-60) Rockhampton, QLD, Australia: Central Queensland University.

Johnston, Stephen, Mucci, Al and Ellis, William (2013). The role of captive koalas in koala conservation. Conserving central Queensland's koalas. (pp. 54-59) edited by Nicole Flint and Alistair Melzer. Rockhampton, QLD, Australia: Central Queensland University.

Gosálvez, J., López-Fernández, C., Arroyo, F., Gosálbez, A., Gutiérrez-Cortés, E. I. and Johnston, S. D. (2013). The assessment of sperm DNA damage in the rabbit using the halomax assay. Rabbits: Biology, Diet and Eating Habits and Disorders. (pp. 87-100) edited by Gianni Adamo and Albert Costanza. Hauppage, NY, U.S.A.: Nova Science Publishers.

Melville, D. F., O'Brien, G. M. and Johnston, S. D. (2011). The novel reproductive biology of the female flying-fox and its implications for the successful development of an artificial insemination programme. The biology and conservation of Australasian bats. (pp. 128-135) edited by Bradley Law, Peggy Eby, Daniel Lunney and Lindy Lumsden. Mosman, NSW Australia: Royal Zoological Society of New South Wales. doi: 10.7882/FS.2011.015

Gosálvez, J., Cortés-Gutiérrez, E. I., Dávila-Rodríguez, M. I., Fernández, J. L., López-Fernández, C. and Johnston, S. D. (2011). Mapping alkali-labile sites in mammalian spermatozoa. Animal reproduction: new research developments. (pp. 219-232) New York, NY, United States: Nova Science Publishers.

Cortés-Gutiérrez, E. I., Dávila-Rodríguez, M. I., Fernández, J. L., Gosálvez, J., Johnston, S. D. and López-Fernández, C. (2009). Mapping alkali-labile sites in mammalian spermatozoa. Animal reproduction : New research developments. (pp. 219-231) edited by Lucas T. Dahnof. New York , NY, U.S.A.: Nova Publishers.

Johnston, S. D. and Holt, W. V. (2001). Germplasm conservation in marsupials. Cryobanking the Genetic Resource. (pp. 201-227) edited by PF Watson and WV Holt. London: Taylor and Francis.

Johnston, S. D., Daddow, L. and Carrick, F. N. (1995). Ultrastructural and light observations of mature spermatozoa and sperm maturation of the Greater Bilby (Macrotis lagotis). Advances in spermatozoal phylogeny and taxonomy. (pp. 397-408) edited by Barrie G M Jamieson, Juan Ausio and Jean-Lou Justine. Paris: Editions du Muséum.

Fenelon, Jane C., Ferrier, Stephanie B., Johnston, Stephen D. and Renfree, Marilyn B. (2024). Observations on the reproductive morphology of the female short-beaked echidna, Tachyglossus aculeatus. Journal of Anatomy. doi: 10.1111/joa.14142

Johnston, Stephen D., Hulse, Lyndal, Keeley, Tamara, Mucci, Albano, Seddon, Jennifer and Maynard, Sam (2024). The utility of the koala scat: a scoping review. Biology, 13 (7) 523, 523. doi: 10.3390/biology13070523

Damyanova, Katerina B., Nixon, Brett, Johnston, Stephen D., Gambini, Andrés, Benitez, Patricio P. and Lord, Tessa (2024). Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation. Biology of Reproduction. doi: 10.1093/biolre/ioae109

Pagliarani, Sara, Johnston, Stephen D., Beagley, Kenneth W. and Palmieri, Chiara (2024). Immunohistochemical characterization of the immune cell response during chlamydial infection in the male and female koala (Phascolarctos cinereus) reproductive tract. Veterinary Pathology, 61 (4), 621-632. doi: 10.1177/03009858231225499

Dutton-Regester, Kate J., Roser, Alice, Meer, Haley, Hill, Andrew, Pyne, Michael, Al-Najjar, Aiman, Whaites, Tim, Fenelon, Jane C., Buchanan, Katherine L., Keeley, Tamara, Renfree, Marilyn B. and Johnston, Stephen D. (2024). Body fat and circulating leptin levels in the captive short-beaked echidna (Tachyglossus aculeatus). Journal of Comparative Physiology B, 194 (4), 1-15. doi: 10.1007/s00360-024-01559-z

Tímermans, Ana, Otero, Fátima, Garrido, Manuel, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2024). The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation, and copy number in normal and abnormal human ejaculates. Andrology, 12 (4), 870-880. doi: 10.1111/andr.13539

Gosálvez, Jaime, Fernández, Carmen López, Johnston, Stephen D. and Bartolomé-Nebreda, Javier (2024). Role of DNase Activity in Human Sperm DNA Fragmentation. Biomolecules, 14 (3) 304, 304. doi: 10.3390/biom14030304

González-Martínez, Mercedes, Sánchez-Martín, Pascual, López-Fernández, Carmen, Johnston, Stephen D. and Gosálvez, Jaime (2024). The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa. Asian Pacific Journal of Reproduction, 13 (1), 22-27. doi: 10.4103/apjr.apjr_42_23

Buthgamuwa, Isini, Fenelon, Jane C., Roser, Alice, Meer, Haley, Johnston, Stephen D. and Dungan, Ashley M. (2023). Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation. MicrobiologyOpen, 12 (6) e1392, e1392. doi: 10.1002/mbo3.1392

Tímermans, Ana, Otero, Fátima, Garrido, Manuel, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2023). Addendum to: The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation and copy number in normal and abnormal human ejaculates. Andrology, 12 (5), 1111-1118. doi: 10.1111/andr.13559

Pagliarani, Sara, Palmieri, Chiara, McGowan, Michael, Carrick, Frank, Boyd, Jackson and Johnston, Stephen D. (2023). Anatomy of the female koala reproductive tract. Biology, 12 (11) 1445, 1-34. doi: 10.3390/biology12111445

Hulse, Lyndal, Tomsett, Caitlin, Roser, Alice, Clark, Lauren, Meer, Haley, Pyne, Michael, Fenelon, Jane C, Renfree, Marilyn B and Johnston, Stephen (2023). An evaluation of DNA sample source and molecular markers to determine gender in the short-beaked echidna (Tachyglossus aculeatus). Zoo Biology, 43 (1), 92-99. doi: 10.1002/zoo.21809

Campbell, Yolande, Palmieri, Chiara, Pagliarani, Sara, Gordon, Jo and Johnston, Stephen (2023). The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation. Biology of Reproduction, 109 (5), 644-653. doi: 10.1093/biolre/ioad098

Fenelon, Jane C., Bennetts, Abbie, Anthwal, Neal, Pyne, Michael, Johnston, Stephen D., Evans, Alistair R., Tucker, Abigail S. and Renfree, Marilyn B. (2023). Getting out of a mammalian egg: the egg tooth and caruncle of the echidna. Developmental Biology, 495, 8-18. doi: 10.1016/j.ydbio.2022.12.005

González-Martínez, Mercedes, López-Fernández, Carmen, Sánchez-Martín, Pascual, Johnston, Stephen D. and Gosálvez, Jaime (2023). Intra-individual variation of sperm DNA fragmentation in the Human ejaculate. Systems Biology in Reproductive Medicine, 69 (3), 215-222. doi: 10.1080/19396368.2023.2175738

Cabello, Yolanda, Belchín, Pedro, González-Martínez, Mercedes, López-Fernández, Carmen, Johnston, Stephen and Gosálvez, Jaime (2023). The efficacy of novel centrifugation-free sperm selection (IO-Lix) on sperm parameters and ICSI reproductive outcomes. Reproductive Biomedicine Online, 46 (2), 267-273. doi: 10.1016/j.rbmo.2022.11.002

Gosálvez, Jaime, Bartolomé-Nebreda, Javier, Tvrdá, Eva, López-Fernández, Carmen, Fernández, José Luís and Johnston, Stephen (2023). Bacterial DNase activity as a putative inductor of sperm DNA fragmentation in infected bull frozen-thawed semen samples. Theriogenology, 197, 224-231. doi: 10.1016/j.theriogenology.2022.12.011

Dutton-Regester, Kate J., Roser, Alice, Meer, Haley, Russell, Freya A., Pyne, Michael, Renfree, Marilyn B., Johnston, Stephen D. and Keeley, Tamara (2023). Investigating the utility of using fecal hormone metabolites as a reproductive management tool for captive short-beaked echidnas (Tachyglossus aculeatus). General and Comparative Endocrinology, 330 114142, 114142. doi: 10.1016/j.ygcen.2022.114142

Russell, Freya A., Johnston, Stephen D., Hill, Andrew, Roser, Alice, Meer, Haley, Fenelon, Jane C., Renfree, Marilyn B. and Keeley, Tamara (2022). Validation of a Non-Invasive Assessment Technique for Quantifying Faecal Glucocorticoid Metabolite Concentrations in The Short-Beaked Echidna (Tachyglossus aculeatus). General and Comparative Endocrinology, 327 114092, 1-8. doi: 10.1016/j.ygcen.2022.114092

Joyce, Briony A., Blyton, Michaela D. J., Johnston, Stephen D., Meikle, William D., Vinette Herrin, Kimberly, Madden, Claire, Young, Paul R. and Chappell, Keith J. (2022). Diversity and transmission of koala retrovirus: a case study in three captive koala populations. Scientific Reports, 12 (1) 15787, 1-8. doi: 10.1038/s41598-022-18939-6

Dutton‐Regester, Kate J., Roser, Alice, Meer, Haley, Renfree, Marilyn B., Johnston, Stephen D. and Keeley, Tamara (2022). Optimizing captive short‐beaked echidna (Tachyglossus aculeatus) fecal sample identification and hormonal analysis. Zoo Biology, 42 (2), 322-327. doi: 10.1002/zoo.21732

Pagliarani, Sara, Johnston, Stephen D., Beagley, Kenneth W., Hulse, Lyndal and Palmieri, Chiara (2022). Chlamydiosis and cystic dilatation of the ovarian bursa in the female koala (Phascolarctos cinereus): Novel insights into the pathogenesis and mechanisms of formation. Theriogenology, 189, 280-289. doi: 10.1016/j.theriogenology.2022.06.022

Hogan, Lindsay A., McGowan, Michael R., Johnston, Stephen D., Lisle, Allan T. and Schooley, Kylie (2022). Suckling behaviour of beef calves during the first five days postpartum. Ruminants, 2 (3), 321-340. doi: 10.3390/ruminants2030022

Gosálvez, Jaime, Vargas‐Baquero, Eduardo, López‐Fernández, Carmen, Bartolomé‐Nebreda, Javier, Fernández, José Luís and Johnston, Stephen (2022). Sperm dna damage in men with spinal cord injury: the relative incidence of single‐ and double‐strand dna breaks. Andrology, 10 (7), 1292-1301. doi: 10.1111/andr.13210

Keating, Shannon E., Fenelon, Jane C., Pyne, Michael, Pinto, Brendan J., Guzmán-Méndez, Irán Andira, Johnston, Stephen D., Renfree, Marilyn B. and Gamble, Tony (2022). Correction to: genetic sex test for the short-beaked echidna (Tachyglossus aculeatus). Conservation Genetics Resources, 14 (3), 279-279. doi: 10.1007/s12686-022-01277-0

Keating, Shannon E., Fenelon, Jane C., Pyne, Michael, Pinto, Brendan J., Guzmán-Méndez, Irán Andira, Johnston, Stephen D., Renfree, Marilyn B. and Gamble, Tony (2022). Genetic sex test for the short-beaked echidna (Tachyglossus aculeatus). Conservation Genetics Resources, 14 (3), 271-278. doi: 10.1007/s12686-022-01258-3

Howell, Lachlan G., Johnston, Stephen D., O’Brien, Justine K., Frankham, Richard, Rodger, John C., Ryan, Shelby A., Beranek, Chad T., Clulow, John, Hudson, Donald S. and Witt, Ryan R. (2022). Modelling genetic benefits and financial costs of integrating biobanking into the captive management of koalas. Animals, 12 (8) 990, 990. doi: 10.3390/ani12080990

Hulse, Lyndal, Palmieri, Chiara, Beagley, Kenneth W., Larkin, Rebecca, Keeley, Tamara, Gosalvez, Jaime and Johnston, Stephen D. (2022). Investigation of pathology associated with Chlamydia pecorum infection in the male reproductive tract, and the effect on spermatogenesis and semen quality in the koala (Phascolarctos cinereus). Theriogenology, 180, 30-39. doi: 10.1016/j.theriogenology.2021.12.011

Tímermans, Ana, Vázquez, Rosana, Otero, Fátima, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2022). Antibiotic toxicity on human spermatozoa assessed using the sperm DNA fragmentation dynamic assay. Andrologia, 54 (2) e14328, e14328. doi: 10.1111/and.14328

Hulse, Lyndal, Beagley, Kenneth W., Larkin, Rebecca and Johnston, Stephen D. (2022). In vitro transmission of Chlamydia using naturally infected koala (Phascolarctos cinereus) semen. Reproduction, Fertility and Development, 34 (3), 378-384. doi: 10.1071/rd21233

Dutton-Regester, Kate J., Roser, Alice, Meer, Haley, Renfree, Marilyn B., Phillips, Clive and Johnston, Stephen D. (2022). Reproductive behaviour before and after oestrus and oviposition in the captive short-beaked echidna (Tachyglossus aculeatus). Reproduction, Fertility and Development, 34 (14), 920-932. doi: 10.1071/rd22092

Adam, D., Johnston, S. D., Beard, L., Nicolson, V., Lisle, A. T., Gaughan, J. B., Larkin, R., Theilemann, P. and Ellis, W. (2022). Temporal effect of feeding on the body temperature and behaviour of captive koalas (Phascolarctos cinereus). Australian Mammalogy, 44 (1), 16-23. doi: 10.1071/AM20024

Baratas, Alejandro, Gosalvez, Jaime, de la Casa, Moises, Camacho, Silvia, Dorado-Silva, Monica, Johnston, Stephen D. and Roy, Rosa (2021). Cumulus cell DNA damage as an Index of human oocyte competence. Reproductive Sciences, 29 (11), 1-7. doi: 10.1007/s43032-021-00817-7

Bartolomé, Javier, Romeo, Sandra Claver, Dorado-Silva, Mónica, García de la Vega, Carlos, López, Carmen, Sánchez-Martín, Pascual, Johnston, Stephen and Gosálvez, Jaime (2021). DNase activity in human seminal plasma and follicular fluid and its inhibition by follicular fluid chelating agents. Reproductive BioMedicine Online, 43 (6), 1079-1086. doi: 10.1016/j.rbmo.2021.09.015

Joyce, Briony A., Blyton, Michaela D. J., Johnston, Stephen D., Young, Paul R. and Chappell, Keith J. (2021). Koala retrovirus genetic diversity and transmission dynamics within captive koala populations. National Academy of Sciences. Proceedings, 118 (38) e2024021118, e2024021118. doi: 10.1073/pnas.2024021118

Skerrett-Byrne, David A., Anderson, Amanda L., Hulse, Lyndal, Wass, Caillin, Dun, Matthew D., Bromfield, Elizabeth G., De Iuliis, Geoffry N., Pyne, Michael, Nicolson, Vere, Johnston, Stephen D. and Nixon, Brett (2021). Proteomic analysis of koala (phascolarctos cinereus) spermatozoa and prostatic bodies. Proteomics, 21 (19) 2100067, e2100067. doi: 10.1002/pmic.202100067

Dutton-Regester, Kate, Keeley, Tamara, Fenelon, Jane C., Roser, Alice, Meer, Haley, Hill, Andrew, Pyne, Michael, Renfree, Marilyn B. and Johnston, Stephen (2021). Plasma progesterone secretion during gestation of the captive short-beaked echidna. Reproduction, 162 (4), 267-275. doi: 10.1530/REP-21-0110

Hulse, Lyndal, Beagley, Kenneth, Larkin, Rebecca, Nicolson, Vere, Gosálvez, Jaime and Johnston, Stephen (2021). The effect of Chlamydia infection on koala (Phascolarctos cinereus) semen quality. Theriogenology, 167, 99-110. doi: 10.1016/j.theriogenology.2021.03.016

Miller, R. R., Beranek, F., Anderson, A. L., Johnston, S. D. and Nixon, B. (2021). Plasma and acrosomal membrane lipid content of saltwater crocodile spermatozoa. Reproduction, Fertility and Development, 33 (9), 596-604. doi: 10.1071/rd21007

Fenelon, Jane C., McElrea, Caleb, Shaw, Geoff, Evans, Alistair R., Pyne, Michael, Johnston, Stephen D. and Renfree, Marilyn B. (2021). The unique penile morphology of the short-beaked echidna, Tachyglossus aculeatus. Sexual Development, 15 (4), 1-10. doi: 10.1159/000515145

Nixon, Brett, Anderson, Amanda L., Bromfield, Elizabeth G., Martin, Jacinta H., Lord, Tessa, Cafe, Shenae L., Roman, Shaun D., Skerrett-Byrne, David A., Eamens, Andrew L., De Iuliis, Geoffry N. and Johnston, Stephen D. (2021). Gross and microanatomy of the male reproductive duct system of the saltwater crocodile Crocodylus porosus. Reproduction, Fertility and Development, 33 (9), 540-554. doi: 10.1071/rd20303

Wallage, Andrea L., Lees, A. M., Lisle, A. T., Lees, J. C., Johnston, S. D. and Gaughan, J. B. (2021). Thermoregulation of the bovine scrotum 2: simulated acute and chronic heat waves reduces the scrotal thermoregulatory capability of Wagyu bulls. International Journal of Biometeorology, 66 (2), 251-262. doi: 10.1007/s00484-021-02108-x

Nixon, Brett, Anderson, Amanda L., Bromfield, Elizabeth G., Martin, Jacinta H., Cafe, Shenae L., Skerrett-Byrne, David A., Dun, Matthew D., Eamens, Andrew L., De Iuliis, Geoffry N. and Johnston, Stephen D. (2021). Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility. Reproduction, Fertility and Development, 33 (9), 530-539. doi: 10.1071/rd20204

Zhou, Yang, Shearwin-Whyatt, Linda, Li, Jing, Song, Zhenzhen, Hayakawa, Takashi, Stevens, David, Fenelon, Jane C., Peel, Emma, Cheng, Yuanyuan, Pajpach, Filip, Bradley, Natasha, Suzuki, Hikoyu, Nikaido, Masato, Damas, Joana, Daish, Tasman, Perry, Tahlia, Zhu, Zexian, Geng, Yuncong, Rhie, Arang, Sims, Ying, Wood, Jonathan, Haase, Bettina, Mountcastle, Jacquelyn, Fedrigo, Olivier, Li, Qiye, Yang, Huanming, Wang, Jian, Johnston, Stephen D., Phillippy, Adam M. ... Zhang, Guojie (2021). Platypus and echidna genomes reveal mammalian biology and evolution. Nature, 592 (7856), 756-762. doi: 10.1038/s41586-020-03039-0

Johnston, Stephen D., Lever, John, McLeod, Robby, Qualischefski, Edward, Madrigal-Valverde, Monica and Nixon, Brett (2021). Assisted breeding technology in the saltwater crocodile Crocodylus porosus: a review and look to the future. Reproduction, Fertility and Development, 33 (9), 503-518. doi: 10.1071/rd20217

Gosálvez, Jaime, López‐Fernández, Carmen, Fernández, José Luís and Johnston, Stephen (2020). Microencapsulation of human spermatozoa increases membrane stability and DNA longevity. Andrologia, 53 (2) e13924, e13924. doi: 10.1111/and.13924

Crespo, F., Wilson, R., Díaz-Jimenez, M., Consuegra, C., Dorado, J., Barrado, B. García, Gosálvez, J., Smit, R. Louis, Hidalgo, M. and Johnston, S. (2020). Effect of season on individual stallion semen characteristics. Animal Reproduction Science, 223 106641, 106641. doi: 10.1016/j.anireprosci.2020.106641

Pagliarani, Sara, Johnston, Stephen D., Beagley, Kenneth W., Dief, Hamdy and Palmieri, Chiara (2020). The occurrence and pathology of chlamydiosis in the male reproductive tract of non-human mammals: a review. Theriogenology, 154, 152-160. doi: 10.1016/j.theriogenology.2020.05.033

Bartolomé-Nebreda, Javier, Vargas-Baquero, Eduardo, López-Fernández, Carmen, Fernández, José Luís, Johnston, Stephen and Gosálvez, Jaime (2020). Free circulating DNA and DNase activity in the ejaculates of men with spinal cord injury. Spinal Cord, 59 (2), 167-174. doi: 10.1038/s41393-020-0518-3

Anthwal, Neal, Fenelon, Jane Catherine, Johnston, Stephen D., Renfree, Marilyn B. and Tucker, Abigail S (2020). Transient role of the middle ear as a lower jaw support across mammals. eLife, 9 e57860, 1-36. doi: 10.7554/elife.57860

Johnston, Stephen D., López-Fernández, Carmen, Pappin, Eloise, Hampe, Alexandra, Doneley, Robert, Lierz, Michael and Gosálvez, Jaime (2020). Assessment of avian sperm DNA fragmentation using the sperm chromatin dispersion assay. Reproduction, Fertility and Development, 32 (10), 948-952. doi: 10.1071/rd19420

Tímermans, Ana, Vázquez, Rosana, Otero, Fátima, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2020). DNA fragmentation of human spermatozoa: simple assessment of single‐ and double‐strand DNA breaks and their respective dynamic behavioral response. Andrology, 8 (5) andr.12819, 1287-1303. doi: 10.1111/andr.12819

Adam, D., Johnston, S. D., Beard, L., Nicolson, V., Gaughan, J. B., Lisle, A. T., FitzGibbon, S., Barth, B. J., Gillett, A., Grigg, G. and Ellis, W. (2020). Body temperature of free-ranging koalas (Phascolarctos cinereus) in south-east Queensland. International Journal of Biometeorology, 64 (8), 1305-1318. doi: 10.1007/s00484-020-01907-y

Vargas-Baquero, Eduardo, Johnston, Stephen, Sánchez-Ramos, Antonio, Arévalo-Martín, Angel, Wilson, Richard and Gosálvez, Jaime (2020). The incidence and etiology of sperm DNA fragmentation in the ejaculates of males with spinal cord injuries. Spinal Cord, 58 (7), 803-810. doi: 10.1038/s41393-020-0426-6

Swinston, Paige, Johnston, Stephen D., O'Hara, Patricia, Englebright, Robert and Keeley, Tamara (2020). Preliminary evaluation of urinary cytology and running wheel activity to detect oestrus and the effect of daily handling on breeding success of fat-tailed dunnarts Sminthopsis crassicaudata. Reproduction, Fertility and Development, 32 (13), 1108-1115. doi: 10.1071/rd20001

Hulse, Lyndal S., Beagley, Kenneth, Ellis, William, Fitzgibbon, Sean, Gillett, Amber, Barth, Ben, Robbins, Amy, Pyne, Michael, Larkin, Rebecca and Johnston, Stephen D. (2020). Epidemiology of chlamydia-induced reproductive disease in male koalas (Phascolarctos cinereus) from Southeast Queensland, Australia as assessed from penile urethral swabs and semen. Journal of Wildlife Diseases, 56 (1) 2019-03-062, 82-92. doi: 10.7589/2019-03-062

Dorado-Silva, Monica, Bartolomé-Nebreda, Javier, Sánchez-Martín, Pascual, Johnston, Stephen and Gosálvez, Jaime (2019). Co-incubation of spermatozoa with human follicular fluid reduces sperm DNA fragmentation by mitigating DNase activity in the seminal plasma. Journal of Assisted Reproduction and Genetics, 37 (1), 63-69. doi: 10.1007/s10815-019-01643-2

Weisbecker, Vera, Guillerme, Thomas, Speck, Cruise, Sherratt, Emma, Abraha, Hyab Mehari, Sharp, Alana C., Terhune, Claire E., Collins, Simon, Johnston, Stephen and Panagiotopoulou, Olga (2019). Individual variation of the masticatory system dominates 3D skull shape in the herbivory-adapted marsupial wombats. Frontiers in Zoology, 16 (1) 41, 41. doi: 10.1186/s12983-019-0338-5

Yu, Tianxiong, Koppetsch, Birgit S, Pagliarani, Sara, Johnston, Stephen, Silverstein, Noah J, Luban, Jeremy, Chappell, Keith, Weng, Zhiping and Theurkauf, William E (2019). The piRNA response to retroviral invasion of the koala genome. Cell, 179 (3), 632-643.e12. doi: 10.1016/j.cell.2019.09.002

Hulse, Lyndal S., McDonald, Sean, Johnston, Stephen D. and Beagley, Kenneth W. (2019). Rapid point‐of‐care diagnostics for the detection of Chlamydia pecorum in koalas ( Phascolarctos cinereus ) using loop‐mediated isothermal amplification without nucleic acid purification. MicrobiologyOpen, 8 (12) e916, e916. doi: 10.1002/mbo3.916

Johnston, S. D., López-Fernández, C., Arroyo, F., Roy, R., Holt, W. V. and Gosálvez, J. (2019). Protamine composition of koala and wombat spermatozoa provides new insights into DNA stability following cryopreservation. Reproduction, Fertility and Development, 31 (10), 1558-1566. doi: 10.1071/rd18512

Valverde, Anthony, Madrigal-Valverde, Mónica, Castro-Morales, Olivier, Gadea-Rivas, Arnoldo, Johnston, Stephen and Soler, Carles (2019). Kinematic and head morphometric characterisation of spermatozoa from the Brown Caiman (Caiman crocodilus fuscus). Animal Reproduction Science, 207, 9-20. doi: 10.1016/j.anireprosci.2019.06.011

Nixon, Brett, Johnston, Stephen D., Skerrett-Byrne, David A., Anderson, Amanda L., Stanger, Simone J., Bromfield, Elizabeth G., Martin, Jacinta H., Hansbro, Philip M. and Dun, Matthew D. (2019). Modification of crocodile spermatozoa refutes the tenet that post-testicular sperm maturation is restricted to mammals. Molecular and Cellular Proteomics, 18 (3), S59-S76. doi: 10.1074/mcp.RA118.000904

Cristescu, Romane H., Miller, Russell L., Schultz, Anthony J., Hulse, Lyndal, Jaccoud, Damian, Johnston, Stephen, Hanger, Jon, Booth, Rosie and Frère, Céline H. (2019). Developing non-invasive methodologies to assess koala population health through detecting Chlamydia from scats. Molecular Ecology Resources, 19 (4) 1755-0998.12999, 957-969. doi: 10.1111/1755-0998.12999

García-Rodríguez, Anaís, Gosálvez, Jaime, Agarwal, Ashok, Roy, Rosa and Johnston, Stephen (2019). DNA damage and repair in human reproductive cells. International Journal of Molecular Sciences, 20 (1) 31, 31. doi: 10.3390/ijms20010031

García Rodríguez, Anaís, de la Casa, Moises, Johnston, Stephen, Gosálvez, Jaime and Roy, Rosa (2019). Association of polymorphisms in genes coding for antioxidant enzymes and human male infertility. Annals of Human Genetics, 83 (1), 63-72. doi: 10.1111/ahg.12286

Pollock, Kate, Gosálvez, Jaime, López-Fernández, Carmen and Johnston, Stephen D. (2018). Amphibian sperm chromatin structure and function and its relevance to sperm preservation. Journal of Herpetology, 52 (4), 486-491. doi: 10.1670/17-163

Feng, Tianyi, Paterson, Brian, Coman, Greg and Johnston, Stephen (2018). Evaluating the impact of moulting and chilled storage of spermatophores on the integrity of plasma membrane, acrosome and DNA of black tiger prawn (Penaeus monodon) spermatozoa. Aquaculture Research, 50 (1), 226-235. doi: 10.1111/are.13888

Palmieri, Chiara, Hulse, Lyndal, Pagliarani, Sara, Larkin, Rebecca, Higgins, Damien P., Beagley, Kenneth and Johnston, Stephen (2018). Chlamydia pecorum infection in the male reproductive system of Koalas (Phascolarctos cinereus). Veterinary Pathology, 56 (2), 300985818806963-306. doi: 10.1177/0300985818806963

González Martínez, Mercedes , Sánchez-Martín, Pascual, Dorado-Silva, Mónica, Fernández, José Luís, Girones, Estibaliz, Johnston, Stephen D. and Gosálvez, Jaime (2018). Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation. Journal of Assisted Reproduction and Genetics, 35 (12), 2215-2221. doi: 10.1007/s10815-018-1319-x

Feng, Tianyi, Paterson, Brian, Panagiotopoulou, Olga, Green, Kathryn, Mardon, Karine and Johnston, Stephen (2018). The functional anatomy of external genitalia in the black tiger prawn (Penaeus monodon) studied by micro-computed tomography and scanning electron microscopy. Journal of Morphology, 279 (9), 1346-1354. doi: 10.1002/jmor.20875

Feng, Tianyi, Paterson, Brian and Johnston, Stephen (2018). A morphological study of the male reproductive tract, post-testicular acrosome maturation and spermatophore formation in the black tiger prawn (Penaeus monodon). Journal of Morphology, 279 (9), 1290-1300. doi: 10.1002/jmor.20869

Adam, D., Beard, L., Johnston, S. D., Nicolson, V., Lisle, A., McKinnon, A., Larkin, R., Theilemann, P., Gillett, A., Brackin, K., FitzGibbon, S., Barth, B. and Ellis, W. (2018). Recording body temperature in koalas (Phascolarctos cinereus): a comparison of techniques. Australian Veterinary Journal, 96 (8), 308-311. doi: 10.1111/avj.12719

Swinbourne, Alyce M., Phillips, Clive J. C., Janssen, Tina, Lisle, Allan, Keeley, Tamara and Johnston, Stephen D. (2018). Reproductive biology of captive female southern hairy-nosed wombats (Lasiorhinus latifrons). Part 2: oestrous behaviour. Reproduction Fertility And Development, 30 (11), 1424-1433. doi: 10.1071/RD17539

Swinbourne, Alyce M., Johnston, Stephen D., Janssen, Tina, Lisle, Alan and Keeley, Tamara (2018). Reproductive biology of captive southern hairy-nosed wombats (Lasiorhinus latifrons). Part 1: oestrous cycle characterisation. Reproduction, Fertility And Development, 30 (11), 1412-1423. doi: 10.1071/RD17497

Hulse, Lyndal S., Hickey, Danica, Mitchell, Jessica M., Beagley, Kenneth W., Ellis, William and Johnston, Stephen D. (2018). Development and application of two multiplex real-time PCR assays for detection and speciation of bacterial pathogens in the koala. Journal of Veterinary Diagnostic Investigation, 30 (4), 1040638718770490-529. doi: 10.1177/1040638718770490

Feng, Tianyi, Gosálvez, Jamie, Lopez-Fernandez, Carmen, Arroyo, Francisca, Paterson, Brian and Johnston, Stephen (2018). Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon. Aquaculture, 491, 281-288. doi: 10.1016/j.aquaculture.2018.03.041

Du, Z., Johnston, S. D., Janssen, T., Phillips, C. J. C., Lisle, A. and Keeley, T. (2018). Behavioral and endocrine responses to season and social dynamics of captive male southern hairy-nosed wombats (Lasiorhinus latifrons). General and Comparative Endocrinology, 261, 40-50. doi: 10.1016/j.ygcen.2018.01.021

Larkin, Rebecca, Palmieri, Chiara, Oishi, Motoharu, Hulse, Lyndal and Johnston, Stephen D. (2018). Ultrasonographic assessment of the male koala (Phascolarctos cinereus) reproductive tract. Research in Veterinary Science, 117, 219-233. doi: 10.1016/j.rvsc.2017.12.019

Swinbourne, A. M., Johnston, S. D., Janssen, T. M., Lisle, A. and Keeley, T. (2017). Validation of techniques to measure reproductive hormones in the urine of female southern hairy-nosed wombats (Lasiorhinus latifrons). General and Comparative Endocrinology, 252, 130-141. doi: 10.1016/j.ygcen.2017.08.003

Du, Z., Keeley, T., Janssen, T., Nicolson, V. and Johnston, S. D. (2017). Measurement of testosterone and cortisol metabolites and luteinising hormone in captive southern hairy-nosed wombat (Lasiorhinus latifrons) urine. General and Comparative Endocrinology, 250, 70-79. doi: 10.1016/j.ygcen.2017.06.001

Sánchez-Martín, Pascual, Dorado-Silva, Mónica, Sánchez-Martín, Fernando, González Martínez, Mercedes, Johnston, Stephen D. and Gosálvez, Jaime (2017). Magnetic cell sorting of semen containing spermatozoa with high DNA fragmentation in ICSI cycles decreases miscarriage rate. Reproductive BioMedicine Online, 34 (5), 506-512. doi: 10.1016/j.rbmo.2017.01.015

Feng, Tianyi, Patterson, Brian and Johnston, Stephen (2017). New insights into the spermatogenesis of the black tiger prawn, Penaeus monodon. Journal of Morphology, 278 (5), 689-703. doi: 10.1002/jmor.20664

Swinbourne, Alyce M., Johnston, Stephen D., Janssen, Tina , Lisle, Allan , Keeley, Tamara and Phillips, Clive J. C. (2017). Olfactory behavioural responses in captive male southern hairy-nosed wombats (Lasiorhinus latifrons) exposed to oestrous and non-oestrous urine samples. Australian Journal of Zoology, 64 (6), 395-401. doi: 10.1071/ZO16088

Wallage, A.L., Gaughan, J.B., Lisle, A.T. , Beard, L., Collins, C.W. and Johnston, S.D. (2017). Measurement of bovine body and scrotal temperature using implanted temperature sensitive radio transmitters, data loggers and infrared thermography. International Journal of Biometeorology, 61 (7), 1-13. doi: 10.1007/s00484-017-1309-1

Wallage, Andrea L., Johnston, S. D., Lisle, A. T., Beard, L., Lees, A. M., Collins, C. W. and Gaughan, J. B. (2017). Thermoregulation of the bovine scrotum 1: measurements of free-range animals in a paddock and pen. International Journal of Biometeorology, 61 (8), 1-7. doi: 10.1007/s00484-017-1315-3

Gosalvez, Jaime, Coppola, Lamberto, Fernandez, Jose Luis, Lopez-Fernandez, Carmen, Gongora, Alfredo, Sanitso, Ricardo, Harrison, Keith, Agarwal, Ashok, Johnston, Stephen and Esteves, Sandro (2017). Multi-centre assessment of nitroblue tetrazolium reactivity in human semen as a potential marker of oxidative stress. Reproductive BioMedicine Online, 34 (5), 513-521. doi: 10.1016/j.rbmo.2017.01.014

Johnston, S. D., Qualischefski, E., Cooper, J., McLeod, R., Lever, J., Nixon, B., Anderson, A. L., Hobbs, R., Gosalvez, J., Lopez-Fernandez, C. and Keeley, T. (2017). Cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa. Reproduction Fertility and Development, 29 (11), 2235-2244. doi: 10.1071/RD16511

Chappell, K. J., Brealey, J. C., Amarilla, A. A., Watterson, D., Hulse, L., Palmieri, C., Johnston, S. D., Holmes, E. C., Meers, J. and Young, P. (2016). Phylogenetic diversity of koala retrovirus within a wild koala population. Journal of Virology, 91 (3) e01820-16, 363-374. doi: 10.1128/JVI.01820-16

Nixon, Brett, Ecroyd, Heath, Dacheux, Jean-Louis, Dacheux, Francoise, Labas, Valerie, Johnston, Stephen D. and Jones, Russell C. (2016). Formation and dissociation of sperm bundles in monotremes. Biology of Reproduction, 95 (4), 1-11. doi: 10.1095/biolreprod.116.140491

Gosalvez, Jaime, Lopez-Fernandez, Carmen and Johnston, Stephen D. (2016). Whole extra-charged DNA spermatozoa in the saltwater crocodile (Crocodylus porous) ejaculate. The Herpetological Journal, 26 (4), 313-316.

Descovich, K. A., Johnston, S., Lisle, A., Nicolson, V., Janssen, T., Brooks, P. and Phillips, C. J. C. (2016). Long-term measurement of body temperature in the southern hairy-nosed wombat (Lasiorhinus latifrons). Australian Mammalogy, 39 (1), 48-55. doi: 10.1071/AM15041

Johnston, Stephen, Lopez-Fernandez, Carmen, Arroyo, Francisca, Gosalbez, Altea, Cortes Gutierrez, Elva I., Fernandez, Jose-Luis and Gosalvez, Jaime (2016). Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model. Journal of Assisted Reproduction and Genetics, 33 (9), 1231-1238. doi: 10.1007/s10815-016-0754-9

Nixon, Brett, Anderson, Amanda L., Smith, Nathan D., McLeod, Robby and Johnston, Stephen D. (2016). The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage. Proceedings of the Royal Society B: Biological Sciences, 283 (1830) 20160495, 20160495. doi: 10.1098/rspb.2016.0495

Schultz, B. K., Palmieri, C., Nicolson, V., Larkin, R., Keeley, T., McGowan, M. and Johnston, S. (2016). First report of a spermatic granuloma and varicocele in a marsupial: a Koala (Phascolarctos cinereus) Case Study. Research in Veterinary Science, 107, 30-33. doi: 10.1016/j.rvsc.2016.05.002

Feng, Tianyi, Paterson, Brian D., Webb, Robyn Webb and Johnston, Stephen D. (2016). Three-dimensional reconstruction of black tiger prawn (Penaeus monodon) spermatozoa using serial block-face scanning electron microscopy. Journal of Morphology, 277 (5), 565-574. doi: 10.1002/jmor.20518

Adam, D., Johnston, S. D., Beard, L., Nicholson, V., Lisle, A., Gaughan, J., Larkin, R., Theilemann, P., Mckinnon, A. and Ellis, W. (2016). Surgical implantation of temperature-sensitive transmitters and data-loggers to record body temperature in koalas (Phascolarctos cinereus). Australian Veterinary Journal, 94 (1-2), 42-47. doi: 10.1111/avj.12393

Crespo, Francisco, Gosálvez, Jaime, Johnston, Stephen D. and de la Torre, Joaquina (2015). Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation. Spanish Journal of Agricultural Research, 13 (4) e04SC02, e04SC02.1-e04SC02.6. doi: 10.5424/sjar/2015134-8108

Johnston, S. D., Lopez-Fernandez, C., Arroyo, F., Fernandez, J. L. and Gosalvez, J. (2015). The assessment of sperm DNA fragmentation in the saltwater crocodile (Crocodylus porosus). Reproduction, Fertility and Development, 29 (3), 630-636. doi: 10.1071/RD15300

Swinbourne, Alyce M., Janssen, Tina, Phillips, Clive J. C. and Johnston, Stephen D. (2015). Non-invasive urine collection in the female southern hairy-nosed wombat (Lasiorhinus latifrons) with the aid of classical conditioning. Zoo Biology, 34 (1), 89-93. doi: 10.1002/zoo.21171

Gosalvez, J., Lopez-Fernandez, C., Fernandez, J.L., Esteves, S.C. and Johnston, S.D. (2015). Unpacking the mysteries of sperm DNA fragmentation: Ten frequently asked questions. Journal of Reproductive Biotechnology and Fertility, 4, 1-16. doi: 10.1177/2058915815594454

Ballantyne, K., Lisle, A., Mucci, A. and Johnston, S. D. (2015). Seasonal oestrous cycle activity of captive female koalas in south-east Queensland. Australian Mammalogy, 37 (2), 245-252. doi: 10.1071/AM14018

Ellis, William, FitzGibbon, Sean, Pye, Geoff, Whipple, Bill, Barth, Ben, Johnston, Stephen, Seddon, Jenny, Melzer, Alistair, Higgins, Damien and Bercovitch, Fred (2015). The role of bioacoustic signals in koala sexual selection: Insights from seasonal patterns of associations revealed with GPSProximity units. PLoS ONE, 10 (7) e0130657, e0130657. doi: 10.1371/journal.pone.0130657

Gosálvez, Jaime, Fernández, José-Luis, Yaniz, Jesus, de la Casa, Moises, López- Fernández, Carmen and Johnston, Stephen (2015). A comparison of sperm DNA damage in the neat ejaculate of sperm donors and males presenting for their initial seminogram. Austin Journal of Reproductive Medicine and Infertility, 2 (3), 1014.1-1014.4.

Ballantyne, K., Anderson, S. T., Mucci, A., Nicolson, V. and Johnston, S. D. (2015). Plasma prolactin concentrations during lactation, pouch young development and the return to behavioural oestrus in captive koalas (Phascolarctos cinereus). Reproduction Fertility and Development, 28 (11), 1838-1843. doi: 10.1071/RD14384

Sanchez-Calabuig, M. J., Lopez-Fernandez, C., Johnston, S. D., Blyde, D., Cooper, J., Harrison, K., de la Fuente, J. and Gosalvez, J. (2015). Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus). Reproduction in Domestic Animals, 50 (2), 227-235. doi: 10.1111/rda.12474

de la Casa, Moises, Fernandez, José Luís, Badajoz, Vicente, Lopez-Fernandez, Carmen, Johnston, Stephen D. and Gosalvez, Jaime (2015). Semi-quantitative assessment of superoxide in the human neat ejaculate using a nitro blue tetrazoilum based assay: a comparison of visual, spectrophotometric and image analysis approaches. Journal of Fertilization: In Vitro, IVF-Worldwide, Reproductive Medicine, Genetics and Stem Cell Biology, 3 (2), 1000146.1-1000146.5. doi: 10.4172/2375-4508.1000146

Johnston, S. D., López-Fernández, C., Arroyo, F., Fardell, S., Roy, R. and Gosálvez, J. (2015). Spermatozoa of Sminthopsis murina (Mammalia: Metatheria) exhibit an unusually high degree of chromatin stability in the absence of disulphide bonding in protamine 1. Reproduction, Fertility and Development, 28 (9), 1268-1275. doi: 10.1071/RD14504

Johnston, S. D., Deif, H. H., McKinnon, A., Theilemann, P., Griffith, J. E. and Higgins, D. P. (2015). Orchitis and epididymitis in koalas (Phascolarctos cinereus) infected with Chlamydia pecorum. Veterinary Pathology, 52 (6), 1254-1257. doi: 10.1177/0300985815570069

Sanchez-Calabuig, M. J., de la Fuente, J., Laguna-Barraza, R., Beltran-Brena, P., Martinez-Nevado, E., Johnston, S. D., Rizos, D., Gutierrez-Adan, A. and Perez-Gutierrez, J. F. (2015). Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa. Theriogenology, 84 (6), 983-994. doi: 10.1016/j.theriogenology.2015.06.001

Wallage, Andrea, Clarke, Lauren, Thomas, Lindy, Pyne, Michael, Beard, Lyn, Ferguson, Arthur, Lisle, Allan and Johnston, Stephen (2015). Advances in the captive breeding and reproductive biology of the short-beaked echidna (Tachyglossus aculeatus). Australian Journal of Zoology, 63 (3), 181-191. doi: 10.1071/ZO14069

Ballantyne, K., Anderson, S. T., Pyne, N., Nicolson, V., Mucci, A., Lisle, A. and Johnston, S. D. (2015). Use of the gonadotrophin-releasing hormone antagonist azaline B to control the oestrous cycle in the koala (Phascolarctos cinereus). Reproduction, Fertility and Development, 28 (11), 1686-1694. doi: 10.1071/RD14349

Ballantyne, K., Anderson, S. T., Pyne, N., Nicolson, V., Mucci, A., Lisle, A. and Johnston, S. D. (2015). The use of a synthetic progesterone, levonorgestrel (LNG), to control the oestrous cycle in the koala. Reproduction, Fertility and Development, 28 (10), 1526-1532. doi: 10.1071/RD14387

Busby, E. R., Soeta, S., Sherwood, N. M. and Johnston, S. D. (2014). Molecular analysis of the koala reproductive hormones and their receptors: gonadotrophin-releasing hormone (GnRH), follicle-stimulating hormone β and luteinising hormone β with localisation of GnRH. Journal of Neuroendocrinology, 26 (12), 870-887. doi: 10.1111/jne.12208

Cortés-Gutiérrez, Elva I., López-Fernández, Carmen, Fernández, José Luis, Dávila-Rodríguez, Martha I., Johnston, Stephen D. and Gosálvez, Jaime (2014). Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay. Frontiers in Genetics, 5 (NOV) 404, 404.1-404.11. doi: 10.3389/fgene.2014.00404

Sanchez-Calabuig, M-J, Lopez-Fernandez, C., Martinez-Nevado, E., Perez-Gutierrez, J. F., de la Fuente, J., Johnston, S. D., Blyde, D., Harrison, K. and Gosalvez, J. (2014). Validation of a field based chromatin dispersion assay to assess sperm DNA fragmentation in the bottlenose dolphin (Tursiops truncatus). Reproduction in Domestic Animals, 49 (5), 761-768. doi: 10.1111/rda.12364

Melville, D. F., Crichton, E. G. and Johnston, S. D. (2014). Semen collection, ejaculate characteristics and in-vitro manipulation of spermatozoa from six-species of captive flying-fox (Pteropus spp.). Reproduction, Fertility and Development, 27 (8), 1233-1241. doi: 10.1071/RD13391

Gosalvez, Jaime, Johnston, Stephen, Lopez-Fernandez, Carmen, Gosalbez, Altea, Arroyo, Francisca, Fernandez, Jose Luis and Alvarez, Juan G. (2014). Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity. Asian Pacific Journal of Reproduction, 3 (2), 116-120. doi: 10.1016/S2305-0500(14)60012-2

Pollock, K., Gosalvez, J., Arroyo, F., Lopez-Fernandez, C., Guille, M., Noble, A. and Johnston, S. D. (2014). Validation of the sperm chromatin dispersion (SCD) test in the Amphibian Xenopus laevis using in situ nick translation and comet assay. Reproduction, Fertility and Development, 27 (8), 1168-1174. doi: 10.1071/RD14070

Cowan, Melinda Lee, Martin, Graeme Bruce, Monks, Deborah Jane, Johnston, Stephen Douglas, Doneley, Robert James Tyson and Blackberry, Margaret Anne (2014). Inhibition of the reproductive system by deslorelin in male and female pigeons (Columba livia). Journal of Avian Medicine and Surgery, 28 (2), 102-108. doi: 10.1647/2013-027

Johnston, S. D., Lever, J., McLeod, R., Qualischefski, E., Brabazon, S., Walton, S. and Collins, S. N. (2014). Extension, osmotic tolerance and cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa. Aquaculture, 426-427, 213-221. doi: 10.1016/j.aquaculture.2014.02.014

Ellis, William, Attard, Rachel, Johnston, Stephen, Theileman, Peter, McKinnon, Allan and Booth, David (2014). Faecal particle size and tooth wear of the koala (Phascolartcos cinereus). Australian Mammalogy, Online Early (1), 1-5. doi: 10.1071/AM13033

Johnston, S. D., Lever, J., McLeod, R., Oishi, M., Qualischefski, E., Omanga, C., Leitner, M., Price, R., Barker, L., Kamau, K., Gaughan, J. and D'Occhio, M. (2014). Semen collection and seminal characteristics of the Australian saltwater crocodile (Crocodylus porosus). Aquaculture, 422-423, 25-35. doi: 10.1016/j.aquaculture.2013.11.002

Seddon, Jennifer M., Lee, Kristen E., Johnston, Stephen D., Nicolson, Vere N., Pyne, Michael, Carrick, Frank N. and Ellis, William A. H. (2014). Testing the regional genetic representativeness of captive koala populations in South-East Queensland. Wildlife Research, 41 (4), 277-286. doi: 10.1071/WR13103

Hogan, Lindsay A., Janssen, Tina and Johnston, Stephen D. (2013). Wombat reproduction (Marsupialia; Vombatidae): an update and future directions for the development of artificial breeding technology. Reproduction, 145 (6), R157-R173. doi: 10.1530/REP-13-0012

Hogan, Lindsay A., Lisle, Allan T. and Johnston, Stephen D. (2013). Eliminative behavior of captive numbats, Myrmecobius fasciatus (Mammalia; Marsupialia): pattern and Identification of fecal deposits. Zoo Biology, 32 (3), 324-331. doi: 10.1002/zoo.21061

Lee, Kristen E., Ellis, William A.H., Carrick, Frank N., Corley, Sean W., Johnston, Stephen D., Baverstock, Peter R., Nock, Catherine J., Rowe, Kevin C. and Seddon, Jennifer M. (2013). Anthropogenic changes to the landscape resulted in colonization of koalas in north-east New South Wales, Australia. Austral Ecology, 38 (3), 355-363. doi: 10.1111/j.1442-9993.2012.02414.x

Crespo, F., Gosalvez, J., Gutierrez-Cepeda, L., Serres, C. and Johnston, S. D. (2013). Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation. Reproduction in Domestic Animals, 48 (2), e23-e25. doi: 10.1111/j.1439-0531.2012.02140.x

D'Occhio, Michael J., Hengstberger, Kirsten J., Tutt, Desmond, Holroyd, Richard G., Fordyce, Geoffry, Boe-Hansen, Gry B. and Johnston, Steve D. (2013). Sperm chromatin in beef bulls in tropical environments. Theriogenology, 79 (6), 946-952. doi: 10.1016/j.theriogenology.2013.01.012

Hogan, Lindsay A., Lisle, Allan T., Johnston, Stephen D., Goad, Tammy and Robertson, Helen (2013). Adult and juvenile sex identification in threatened monomorphic Geocrinia frogs using fecal steroid analysis. Journal of Herpetology, 47 (1), 112-118. doi: 10.1670/11-290

Oishi, Motoharu, Takahashi, Mei, Amasaki, Hajime, Janssen, Tina and Johnston, Stephen D. (2013). The seminiferous epithelial cycle and microanatomy of the koala (Phascolarctos cinereus) and southern hairy-nosed wombat (Lasiorhinus latifrons) testis. Journal of Anatomy, 222 (3), 380-389. doi: 10.1111/joa.12020

Descovich, Kristin A., Lisle, Allan T., Johnston, Stephen and Phillips, Clive J. C. (2013). The effect of group size on vigilance in a semi-solitary, fossorial marsupial (Lasiorhinus latifrons). Behavioural Processes, 100, 208-213. doi: 10.1016/j.beproc.2013.09.015

Gosalvez, Jaime, Caballero, Pedro, Lopez-Fernandez, Carmen, Ortega, Leonor, Guijarro, Jose Andres, Fernandez, Jose Luis, Johnston, Stephen D. and Nunez-Calonge, Rocio (2013). Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?. Asian Journal of Andrology, 15 (6), 812-818. doi: 10.1038/aja.2013.74

Johnston, S. D., Booth, R. A., Pyne, M., Keeley, T., Mackie, J. T., Hulse, L. and Ellis, W. (2013). Preliminary study of faecal cortisol and corticosterone as an index of acute cortisol secretion in the koala (Phascolarctos cinereus). Australian Veterinary Journal, 91 (12), 534-537. doi: 10.1111/avj.12125

Ellis, William, FitzGibbon, Sean, Melzer, Alistair, Wilson, Robbie, Johnston, Steve, Berkovitch, Fred, Dique, David and Carrick, Frank (2013). Koala habitat use and population density: using field data to test the assumptions of ecological models. Australian Mammalogy, 35 (2), 160-165. doi: 10.1071/AM12023

Melville, D. F., Johnston, S. D. and Miller Jr., R. R. (2012). Flying-fox (Pteropus spp.) sperm membrane fatty acid composition, its relationship to cold shock injury and implications for cryopreservation success. Cryobiology, 65 (3), 224-229. doi: 10.1016/j.cryobiol.2012.06.007

Wells, R., Kenny, A. L., Duckett, R., Wreford, N. G., Johnston, S. D. and D'Occhio, M. J. (2012). Elucidation of the role of LH and FSH during neonatal testicular development and growth in the boar. Animal Reproduction Science, 137 (1-2), 74-81. doi: 10.1016/j.anireprosci.2012.11.005

Lovas, Erica M., Filippich, Lucio J. and Johnston, Stephen D. (2012). Spermiogenesis in the Australian Cockatiel Nymphicus hollandicus. Journal of Morphology, 273 (11), 1291-1305. doi: 10.1002/jmor.20060

Nunez-Calonge, Rocio, Caballero, Pedro, Lopez-Fernandez, Carmen, Andres Guijarro, J., Luis Fernandez, Jose, Johnston, Stephen and Gosalvez, Jaime (2012). An improved experimental model for understanding the impact of sperm DNA fragmentation on human pregnancy following ICSI. Reproductive Sciences, 19 (11), 1163-1168. doi: 10.1177/1933719112459238

Hogan, Lindsay A., Lisle, Allan T., Johnston, Stephen D. and Robertson, Helen (2012). Non-invasive assessment of stress in captive numbats, Myrmecobius fasciatus (Mammalia: Marsupialia), using faecal cortisol measurement. General and Comparative Endocrinology, 179 (3), 376-383. doi: 10.1016/j.ygcen.2012.09.020

Johnston, Stephen D., Zee, Yeng Peng, Lopez-Fernandez, Carmen and Gosalvez, Jamie (2012). The effect of chilled storage and cryopreservation on the sperm DNA fragmentation dynamics of a captive population of koalas. Journal of Andrology, 33 (5), 1007-1015. doi: 10.2164/jandrol.111.015248

Hogan, L. A., Lisle, A. T., Valentine, L., Johnston, S. D. and Robertson, H. (2012). Non-invasive monitoring of male and female numbat (Myrmecobius fasciatus: Myrmecobiidae) reproductive activity. Animal Reproduction Science, 133 (3-4), 237-245. doi: 10.1016/j.anireprosci.2012.07.005

Descovich, Kristin A., Lisle, Allan T., Johnston, Stephen and Phillips, Clive J. C. (2012). Space allowance and the behaviour of captive southern hairy-nosed wombats (Lasiorhinus latifrons). Applied Animal Behaviour Science, 140 (1-2), 92-98. doi: 10.1016/j.applanim.2012.05.009

Santiso, Rebeca, Tamayo, María, Gosalvez, Jaime, Johnston, Steve, Marino, Alfonso, Fernandez, Carlos, Losada, Carlos and Fernandez, Jose Luis (2012). DNA fragmentation dynamics allows the assessment of cryptic sperm damage in human: Evaluation of exposure to ionizing radiation, hyperthermia, acidic pH and nitric oxide. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 734 (1-2), 41-49. doi: 10.1016/j.mrfmmm.2012.03.006

Johnston, S. D., Satake, N., Zee, Y., Lopez-Fernandez, C., Holt, W. V. and Gosalvez, J. (2012). Osmotic stress and cryoinjury of koala sperm: An integrative study of the plasma membrane, chromatin stability and mitochondrial function. Reproduction, 143 (6), 787-797. doi: 10.1530/REP-11-0436

Descovich, Kristin A., Lisle, Allan T., Johnston, Stephen, Keeley, Tamara and Phillips, Clive J. C. (2012). Intrasample variation and the effect of storage delay on faecal metabolite concentrations in the southern hairy-nosed wombat (Lasiorhinus latifrons). Australian Mammalogy, 34 (2), 217-222. doi: 10.1071/AM11047

Descovich, Kristin A., Lisle, Allan T., Johnston, Stephen, Nicolson, Vere and Phillips, Clive J. C. (2012). Differential responses of captive southern hairy-nosed wombats (Lasiorhinus latifrons) to the presence of faeces from different species and male and female conspecifics. Applied Animal Behaviour Science, 138 (1-2), 110-117. doi: 10.1016/j.applanim.2012.01.017

Melville, D. F., O'Brien, G. M., Crichton, E. G., Theilemann, P., McKinnon, A. and Johnston, S. D. (2012). Reproductive seasonality and the effect of the GnRH agonist deslorelin as a contraceptive in captive male Black Flying-foxes (Pteropus alecto). Theriogenology, 77 (3), 652-661. doi: 10.1016/j.theriogenology.2011.09.012

Lee, Kristen E., Seddon, Jennifer M., Johnston, Stephen, FitzGibbon, Sean I., Carrick, Frank, Melzer, Alistair, Bercovitch, Fred and Ellis, William (2012). Genetic diversity in natural and introduced island populations of koalas in Queensland. Australian Journal of Zoology, 60 (5), 303-310. doi: 10.1071/ZO12075

Hogan, Lindsay A., Johnston, Stephen D., Lisle, Allan T., Keeley, Tamara, Wong, Phoenix, Nicolson, Vere, Horsup, Alan B., Janssen, Tina and Phillips, Clive J. C. (2011). Behavioural and physiological responses of captive wombats (Lasiorhinus latifrons) to regular handling by humans. Applied Animal Behaviour Science, 134 (3-4), 217-228. doi: 10.1016/j.applanim.2011.07.010

Hogan, L.A., Phillips, C.J.C., Horsup, A.B., Janssen, T. and Johnston, S.D. (2011). Technique for faecal marking in group-housed southern hairy-nosed wombats Lasiorhinus latifrons. Australian Zoologist, 35 (3), 649-654. doi: 10.7882/AZ.2011.017

López-Fernández, C., Johnston, S. D., Gosálbez, A. and Gosálvez, J. (2011). Seasonal changes in sperm DNA fragmentation of Murciano-Granadina goats: The compelling case for dynamic assessment. Small Ruminant Research, 100 (1), 50-53. doi: 10.1016/j.smallrumres.2011.05.006

Enciso, M., Cisale, H., Johnston, S.D., Sarasa, J., Fernandez, J.L. and Gosalvez, J. (2011). Major morphological sperm abnormalities in the bull are related to sperm DNA damage. Theriogenology, 76 (1), 23-32. doi: 10.1016/j.theriogenology.2010.12.034

Garcia-Contreras, Adelfa, De Loera, Yasmin, Garcia-Artiga, Carlos, Palomo, Antonio, Guevara, Jesús A., Herrera-Haro, José, Lopez-Fernandez, Carmen, Johnston, Steve and Gosalvez, Jaime (2011). Elevated dietary intake of Zn-methionate is associated with increased sperm DNA fragmentation in the boar. Reproductive Toxicology, 31 (4), 570-573. doi: 10.1016/j.reprotox.2010.12.003

Enciso, Maria, Johnston, Stephen D. and Gosalvez, Jaime (2011). Differential resistance of mammalian sperm chromatin to oxidative stress as assessed by a two-tailed comet assay. Reproduction Fertility and Development, 23 (5), 633-637. doi: 10.1071/RD10269

Hogan, Lindsay A., Johnston, Steve D., Lisle, Allan T., Horsup, Alan B., Janssen, Tina and Phillips, Clive J. C. (2011). The effect of environmental variables on the activity patterns of the southern hairy-nosed wombat (Lasiorhinus latifrons) in captivity: Onset, duration and cessation of activity. Australian Journal of Zoology, 59 (1), 35-41. doi: 10.1071/ZO11006

Lee, Kristen E., Seddon, Jennifer M., Corley, Sean W., Ellis, William A. H., Johnston, Stephen D., de Villiers, Deidré L., Preece, Harriet J. and Carrick, Frank N. (2010). Genetic variation and structuring in the threatened koala populations of Southeast Queensland. Conservation Genetics, 11 (6), 2091-2103. doi: 10.1007/s10592-009-9987-9

Lopez-Fernandez, C., Johnston, S. D., Fernandez, J. L., Wilson, R. J. and Gosalvez, J. (2010). Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration. Theriogenology, 74 (8), 1362-1370. doi: 10.1016/j.theriogenology.2010.06.006

Muller, Tracy L., Ensabella, Traza J., Booth, Rosie, Johnston, Stephen D. and Phillips, Clive J. C. (2010). The behaviour and environmental enrichment of captive mahogany gliders (Petaurus gracilis). Australian Mammalogy, 32 (2), 109-116. doi: 10.1071/AM09035

Hogan, LA, Johnston, SD, Lisle, A, Horsup, AB, Janssen, T and Phillips, CJC (2010). Stereotypies and environmental enrichment in captive southern hairy-nosed wombats, Lasiorhinus latifrons. Applied Animal Behaviour Science, 126 (1-2), 85-95. doi: 10.1016/j.applanim.2010.05.009

Hogan, L. A., Phillips, C. J. C., Lisle, A., Keeley, T., Horsup, A. B., Janssen, T. and Johnston, S. D. (2010). Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons). Animal Reproduction Science, 119 (3-4), 293-304. doi: 10.1016/j.anireprosci.2010.01.006

Johnston, S. D., Rumph, C., Lucht, M., Stenzel, D., Nicolson, V., Blyde, D. and Keeley, T. (2010). Studies of male reproduction in the Greater Bilby Macrotis lagotis. Australian Zoologist, 35 (2), 315-330. doi: 10.7882/AZ.2010.020

Hogan, L. A., Phillips, C. J. C., Horsup, A. B., Keeley, T., Nicolson, V., Janssen, T., Lisle, A. and Johnston, S. D. (2010). Monitoring male southern hairy-nosed wombat (Lasiorhinus latifrons) reproductive function and seasonality in a captive population. Animal Reproduction Science, 118 (2-4), 377-387. doi: 10.1016/j.anireprosci.2009.10.001

Allen, Camryn D., de Villiers, Deidre L., Manning, Brett D., Dique, David S., Burridge, Michelle, Chafer, Mandy L., Nicolson, Vere N., Jago, Sophia C., McKinnon, Allan J., Booth, Rosemary J., Mckee, Jeff J., Pyne, Michael J., Zee, Yeng P., Lundie-Jenkins, Geoff, Theilemann, Peter, Wilson, Richard J., Carrick, Frank N. and Johnston, Stephen D. (2010). Seasonal reproduction in wild and captive male koala (Phascolarctos cinereus) populations in south-east Queensland. Reproduction Fertility and Development, 22 (4), 695-709. doi: 10.1071/RD09113

Hogan, Lindsay, Phillips, Clive, Lisle, Allan, Horsup, Alan, Janssen, Tina and Johnston, Stephen (2010). Reproductive behaviour of the southern-hairy nosed wombat (Lasiorhinus latifrons). Australian Journal of Zoology, 58 (6), 350-361. doi: 10.1071/ZO10068

Pollock, K., Booth, R., Wilson, R., Keeley, T., Grogan, K., Kennerley, P. and Johnston, S. D. (2010). Oestrus in the Julia Creek dunnart (Sminthopsis douglasi) is associated with wheel running behaviour but not necessarily changes in body weight, food consumption or pouch morphology. Animal Reproduction Science, 117 (1-2), 135-146. doi: 10.1016/j.anireprosci.2009.03.005

Lovas, E. M., Johnston, S. D. and Filippich, L. J. (2010). Using a GnRH agonist to obtain an index of testosterone secretory capacity in the cockatiel (Nymphicus hollandicus) and sulphur-crested cockatoo (Cacatua galerita). Australian Veterinary Journal, 88 (1-2), 52-56. doi: 10.1111/j.1751-0813.2009.00533.x

Hogan, Lindsay A., Phillips, Clive J. C., Lisle, Allan, Horsup, Alan B., Janssen, Tina and Johnston, Stephen D. (2009). Remote monitoring of the behaviour and activity of captive southern hairy-nosed wombats (Lasiorhinus latifroms). Australian Mammalogy, 31 (2), 123-135. doi: 10.1071/AM09023

Portas, T., Johnston, S.D., Hermes, R., Arroyo, F., Lopez-Fernandez, C., Bryant, B., Hilebrandt, T.B., Goritz, F. and Gosalvez, J. (2009). Frozen-thawed rhinoceros sperm exhibit DNA damage shortly after thawing when assessed by the sperm chromatin dispersion assay. Theriogenology, 72 (5), 711-720. doi: 10.1016/j.theriogenology.2009.05.008

Zee, Y. P., Lopez-Fernandez, C., Arroyo, F., Johnston, S. D., Holt, W. V. and Gosalvez, J. (2009). Evidence that single-stranded DNA breaks are a normal feature of koala sperm chromatin, while double-stranded DNA breaks are indicative of DNA damage. Reproduction, 138 (2), 267-278. doi: 10.1530/REP-09-0021

Zee, Y. P., Holt, W. V., Nicolson, V., Pyne, M. and Johnston, S. D. (2009). Individual variability in post-thaw sperm survival in a captive koala population. Cryobiology, 59 (1), 69-74. doi: 10.1016/j.cryobiol.2009.04.010

Ballantyne, K., Matson, P., Noakes, N., Nicolson, V. and Johnston, S. D. (2009). Reproductive physiology of the female greater bilby (Macrotis lagotis Thylacomyidae): Evidence for a male-induced luteal phase. Reproduction, Fertility and Development, 21 (2), 274-282. doi: 10.1071/RD08134

Johnston, S. D., Lopez-Fernandez, C., Gosalbez, A., Holt, W. V. and Gosalvez, J. (2009). Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata). Reproduction Fertility and Development, 21 (8), 1008-1014. doi: 10.1071/RD09079

Rodger, John C., Paris, Damien B.B.P., Czarny, Natasha A., Harris, Merrilee S., Molinia, Frank C., Taggart, David A., Allen, Camryn D. and Johnston, Stephen D. (2009). Artificial insemination in marsupials. Theriogenology, 71 (1), 176-189. doi: 10.1016/j.theriogenology.2008.09.006

McClean, Rhett, Holt, William V., Zee, Yeng Peng, Lisle, Allan and Johnston, Stephen D. (2008). The effect of cryoprotectant on kangaroo sperm ultrastructure and mitochondrial function. Cryobiology, 57 (3), 297-303. doi: 10.1016/j.cryobiol.2008.08.006

McClean, Rhett, Zee, Yeng Peng, Holt, William V. and Johnston, Stephen D. (2008). Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cycotoxic exposure to glycerol. Cryobiology, 57 (3), 304-307. doi: 10.1016/j.cryobiol.2008.08.007

Zee, Yeng Peng, Holt, William V., Gosalvez, Jaime, Allen, Camryn D., Nicolson, Vere, Pyne, Michael, Burridge, Michelle, Carrick, Frank N. and Johnston, Stephen D. (2008). Dimethylacetamide can be used as an alternative to glycerol for the successful cryopreservation of koala (Phascolarctos cinereus) spermatozoa. Reproduction Fertility and Development, 20 (6), 724-733. doi: 10.1071/RD08036

Johnston, S. D., Camacho, F. C., Carillo, L., Guy, N., Govea, J., Martinez, O., Paras, A., Lisle, A. T. and D'Occhio, M. (2008). The development of a testosterone stimulation test in the virginia opossum (Dedelphis virginiana) and its use in evaluating deslorelin contraception. Reproduction, Fertility and Development, 20 (5), 563-569. doi: 10.1071/RD07215

Phillips, D. J., McKinnon, A., Keeley, T. and Johnston, S. D. (2008). Testosterone secretion, testicular histology and the cryopreservation of cauda epididymidal spermatozoa in the common ringtail possum (Pseduocheirus peregrinus). Reproduction Fertility and Development, 20 (3), 391-401. doi: 10.1071/RD07206

Allen, Camryn D., Burridge, Michelle, Chafer, Mandy L., Nicolson, Vere N., Jago, Sophia C., Booth, Rosemary L., Fraser, Grant, Ensabella, Traza-Jade, Zee, Yeng Peng, Lundie-Jenkins, Geoff, Holt, William V., Lisle, Allan T., Carrick, Frank N., Curlewis, Jonathan D., D'Occhio, Michael J. and Johnston, Stephen D. (2008). Control of the koala (Phascolarctos cinereus) anterior pituitary-godadal axis with analogues of GnRH. Reproduction Fertility And Development, 20 (5), 598-605. doi: 10.1071/RD07214

Wells, R., Scott, P. T., Harrison, D. K., Wreford, N. G., Duckett, R., Johnston, S. D. and D'Occhio, M. J. (2008). Expression of FGF2 and TGFα and testis morphology during testicular hypertrophy subsequent to hemicastration in the neonatal boar. Molecular Reproduction and Development, 75 (6), 961-966. doi: 10.1002/mrd.20743

Debbie F. Melville, Elizabeth G. Crichton, Trish Paterson-Wimberley and Johnston, Stephen D. (2008). Collection of semen by manual stimulation and ejaculate characteristics of the Black Flying-Fox (Pteropus alecto). Zoo Biology, 27 (2), 159-164. doi: 10.1002/zoo.20169

Allen, C. D., Burridge, M., Mulhall, S., Chafer, M. L., Nicolson, V. N., Pyne, M., Zee, Y. P., Jago, S. C., Lundie-Jenkins, G., Holt, W. V., Carrick, F. N., Curlewis, J. D., Lisle, A. T. and Johnston, S. D. (2008). Successful artificial insemination in the koala (Phascolarctos cinereus) using extended and extended-chilled semen collected by electroejaculation. Biology of Reproduction, 78 (4), 661-666. doi: 10.1095/biolreprod.107.064824

Johnston, S. D., Smith, B., Pyne, M., Stenzel, D. and Holt, W. V. (2007). One-sided ejaculation of echidna sperm bundles. American Naturalist, 170 (6), E162-E164. doi: 10.1086/522847

Rens, Willem, O'Brien, Patricia C. M., Grutzner, Frank, Clarke, Oliver, Graphodatskaya, Daria, Tsend-Ayush, Enkhjargal, Trifonov, Vladimir A., Skelton, Helen, Wallis, Mary C., Johnston, Steve, Veyrunes, Frederic, Graves, Jennifer A. M. and Ferguson-Smith, Malcolm A. (2007). The multiple sex chromosomes of platypus and echidna are not completely identical and several share homology with the avian Z. Genome Biology, 8 (11) R243, R243.1-R243.21. doi: 10.1186/gb-2007-8-11-r243

Johnston, Stephen D., Lopez-Fernandez, Carmen, Gosalbez, Altea, Zee, Yeng peng, Holt, William V., Allen, Camryn and Gosalvez, Jaime (2007). The relationship between sperm morphology and chromatin integrity in the koala (Phascolarctos cinereus) as assessed by the sperm chromatin dispersion test (SCDt). Journal of Andrology, 28 (6), 891-899. doi: 10.2164/jandrol.107.003350

Johnston, Stephen D., Ward, D., Lemon, J., Gunn, I., MacCallum, C. A., Keeley, T. and Blyde, D. (2007). Studies of male reproduction in captive African wild dogs (Lycaon pictus). Animal Reproduction Science, 100 (3-4), 338-355. doi: 10.1016/j.anireprosci.2006.08.017

Feige, Stacey, Nilsson, Kate, Phillips, Clive J.C. and Johnston, Steve D. (2007). Heterosexual and homosexual behaviour and vocalisations in captive female koalas (Phascolarctos cinereus). Applied Animal Behaviour Science, 103 (1-2), 131-145. doi: 10.1016/j.applanim.2006.03.014

D'Occhio, M. J., Hengstberger, K. and Johnston, S. D. (2007). Biology of sperm chromatin structure and relationship to male fertility and embryonic survival. Animal Reproduction Science, 101 (1-2), 1-17. doi: 10.1016/j.anireprosci.2007.01.005

Lopez-Fernandez, C., Crespo, F., Arroyo, F., Fernandez, J. L., Arana, P., Johnston, S. D. and Gosalvez, J. (2007). Dynamics of sperm DNA fragmentation in domestic animals - II. The stallion. Theriogenology, 68 (9), 1240-1250. doi: 10.1016/j.theriogenology.2007.08.029

Zee, Y. P., Holt, W. V., Allen, C. D., Nicolson, V., Burridge, M., Lisle, A., Carrick, F. N. and Johnston, S. D. (2007). Effects of cryopreservation on mitochondrial function and heterogeneity, lipid raft stability and phosphatidylserine translocation in koala (Phascolarctos cinereus) spermatozoa. Reproduction Fertility And Development, 19 (7), 850-860. doi: 10.1071/RD07084

de la Torre, J., Lopez-Fernandez, C., Pita, M., Fernandez, J. L., Johnston, S. D. and Gosalvez, J. (2007). Simultaneous observation of DNA fragmentation and protein loss in the boar spermatozoon following application of the sperm chromatin dispersion (SCD) test. Journal of Andrology, 28 (4), 533-540. doi: 10.2164/jandrol.106.002246

McClean, R. V., Holt, W. V. and Johnston, S. D. (2007). Ultrastructural observations of cryoinjury in kangaroo spermatozoa. Cryobiology, 54 (3), 271-280. doi: 10.1016/j.cryobiol.2007.03.004

Johnston, S. D., Nicolson, V., Madden, C., Logie, S., Pyne, M., Roser, A., Lisle, A. T. and D'Occhio, M. (2007). Assessment of reproductive status in male echidnas. Animal Reproduction Science, 97 (1-2), 114-127. doi: 10.1016/j.anireprosci.2005.12.016

van der Straten, K. M., Leung, L. K. P., Rossini, R. and Johnston, S. D. (2006). Cryopreservation of spermatozoa of black marlin, Makaira indica (Teleostei : Istiophoredae). Cryoletters, 27 (4), 203-209.

McClean, R., MacCallum, C., Blyde, D., Hole, W. and Johnston, S. (2006). Actin localisation and the effect of cytochalasin D on the osmotic tolerance of cauda epedidymidal kangaroo spermatozoa. Cryoletters, 27 (4), 253-260.

Allen, Camryn D., McKinnon, Allan J., Lisle, Allan T., D'Occhio, Michael J. and Johnston, Stephen D. (2006). Use of a GnRH agonist and hCG to obtain an index of testosterone secretory capacity in the koala (Phascolarctos cinereus). Journal of Andrology, 27 (6), 720-724. doi: 10.2164/jandrol.106.000117

Johnston, S. D., Madden, C., Nicolson, V. and Pyne, M. (2006). Identifying the sex of short-beaked echidnas. Australian Veterinary Journal, 84 (1-2), 63-65. doi: 10.1111/j.1751-0813.2006.tb13131.x

Johnston, S. D., Madden, C., Nicolson, V., Cowin, G., Pyne, M. and Booth, R. (2006). Venipuncture in the short-beaked echidna. Australian Veterinary Journal, 84 (1-2), 66-67. doi: 10.1111/j.1751-0813.2006.tb13132.x

McClean, Rhett, MacCallum, Catriona, Blyde, David, Holt, William V. and Johnston, Stephen D. (2006). Ultrastructure, osmotic tolerance, glycerol toxicity and cryopreservation of caput and cauda epididymidal kangaroo spermatozoa. Reproduction Fertility And Development, 18 (4), 469-476. doi: 10.1071/RD05148

Ward, David G., Blyde, David, Lemon, John and Johnston, Steve (2006). Anesthesia of captive African wild dogs (Lycaon pictus) using a medetomidine-ketamine-atropine combination. Journal of Zoo And Wildlife Medicine, 37 (2), 160-164. doi: 10.1638/05-037.1

Johnston, S. D., MacCallum, C., Blyde, D., McClean, R., Lisle, A. and Holt, W. V. (2006). An investigation into the similarities and differences governing the cryopreservation success of koala (Phascolarctos cinereus: goldfuss) and common wombat (Vombatus ursinus: shaw) spermatozoa. Cryobiology, 53 (2), 218-228. doi: 10.1016/j.cryobiol.2006.06.001

Iimori, E., D'Occhio, M. J., Lisle, A. T. and Johnston, S. D. (2005). Testosterone secretion and pharmacological spermatozoal recovery in the cane toad (Bufo marinus). Animal Reproduction Science, 90 (1-2), 163-173. doi: 10.1016/j.anireprosci.2005.01.010

de Jong, C. E., Jonsson, N., Field, H., Smith, C., Crichton, E. G., Phillips, N. and Johnston, S. D. (2005). Collection, seminal characteristics and chilled storage of spermatozoa from three species of free-range flying fox (Pteropus spp.). Theriogenology, 64 (5), 1072-1089. doi: 10.1016/j.theriogenology.2005.02.016

MacCallum, C. and Johnston, S. D. (2005). Studies on the cryopreservation of common wombat (Vombatus ursinus) spermatozoa. Reproduction Fertility and Development, 17 (7), 727-732. doi: 10.1071/RD05082

Johnston, S. D., O'Callaghan, P. O., Nilsson, K., Tzipori, G. and Curlewis, J. D. (2004). Semen-induced luteal phase and identification of a LH surge in the koala (Phascolarctos cinereus). Reproduction, 128 (5), 629-634. doi: 10.1530/rep.1.00300

Miller, R. R. Jr, Sheffer, C. J., Cornett, C. L., McClean, R., MacCallum, C. and Johnston, S. D. (2004). Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation success. Cryobiology, 49 (2), 137-148. doi: 10.1016/j.cryobiol.2004.06.002

Bryant, B., Turner, L., MacCallum, C. and Johnston, S. D. (2004). Persistent penile frenulum in a nilgai (Boselaphus tragocamelus). Australian Veterinary Journal, 82 (7), 419-420. doi: 10.1111/j.1751-0813.2004.tb11131.x

Phillips, N. J., McGowan, M. R., Johnston, S. D. and Mayer, D. G. (2004). Relationship between thirty post-thaw spermatozoal characteristics and the field fertility of 11 high-use Australian dairy AI sires. Animal Reproduction Science, 81 (1-2), 47-61. doi: 10.1016/j.anireprosci.2003.10.003

Jonsson, N. N., Johnston, S. D., Field, H., de Jong, C. and Smith, C. (2004). Field anaesthesia of three Australian species of flying fox. Veterinary Record, 154 (21), 664-664. doi: 10.1136/vr.154.21.664

Johnston, S. D., Daddow, L., Carrick, F. N. and Jamieson, B. (2004). Observations of spermiogenesis and epididymal sperm maturation in the rufous hare wallaby, Lagorchestes hirsutus (Metatheria, Mammalia). Acta Zoologica, 85 (1), 53-58. doi: 10.1111/j.0001-7272.2004.00157.x

Phillips, N. J., Mcgowan, M. R., Johnston, S. D. and Mayer, D. G. (2004). Relationship between thirty post-thaw spermatozoal characteristics and the field fertility of 11 high-use Australian dairy AI sires. Animal Reproduction Science, 80 (1-2), 47-61. doi: 10.1016/S0378-4320(03)00137-4

Bodetti, T. J., Hengstberger, K., Johnston, S. and Timms, P. (2003). Evaluation of tetracycline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae. Veterinary Record, 153 (19), 588-591. doi: 10.1136/vr.153.19.588

Johnston, S. D., McGowan, M. R., O'Callaghan, P., Cox, R., Houlden, B., Haig, S. and Taddeo, G. (2003). Birth of Koalas Phascolarctos cinereus at Lone Pine Koala Sanctuary following artificial insemination. International Zoo Yearbook, 38 (1), 160-172. doi: 10.1111/j.1748-1090.2003.tb02076.x

Johnston, S. D., Daddow, L., Carrick, F. N., Jamieson, B. and Smith, L. (2003). Cauda epididymal spermatozoa of the rufous hare wallaby, Lagorchestes hirsutus (Metatheria, Mammalia) imaged by electron and confocal microscopy. Acta Zoologica : morphology and evolution, 84 (2), 139-143. doi: 10.1046/j.1463-6395.2003.00139.x

Bodetti, T. J., Johnston, S., Pospischil, A., Knox, C. and Timms, P. (2002). Screening semen from koalas (Phascolarctos cinereus) for Chlamydia species by PCR. Veterinary Record, 151 (5), 147-149.

Johnston, S. D., McGowan, M. R. and Blyde, D. (2002). Birth of a Banteng (Bos javanicus) calf at Western Plains Zoo after fixed time artificial insemination. Australian Veterinary Journal, 80 (1-2), 94-95. doi: 10.1111/j.1751-0813.2002.tb12848.x

Townsend, K., Yates, R., O'Boyle, D., Johnston S. D. and Blackall, L. (2001). Phylogenetic analysis of a novel Coryneform isolated from koala semen and prepuce. Microbiology Australia, 22, 96.

Johnston, S. D., Blyde, D., Pedrana, R. and Gibbs, A. (2000). Laparoscopic intrauterine insemination in Barbary sheep (Ammotragus lervia). Australian Veterinary Journal, 78 (10), 714-716. doi: 10.1111/j.1751-0813.2000.tb10414.x

Johnston, S. D., Mcgowan, M. R., O'Callaghan, P., Cox, R. and Nicholson, V. (2000). Studies of the oestrous cycle, oestrus and pregnancy in the koala (Phascolarctos cinereus). Journal of Reproduction and Fertility, 120 (1), 49-57. doi: 10.1530/reprod/120.1.49

Johnston, S. D., Mcgowan, M. R., O'Callaghan, P., Cox, R. and Nicholson, V. (2000). Natural and artificial methods for inducing the luteal phase in the koala (Phascolarctos cinereus). Journal of Reproduction and Fertility, 120 (1), 59-64. doi: 10.1530/reprod/120.1.59

Johnston, S. D., Mcgowan, M. R., Phillips, N. and O'Callaghan, P. (2000). Optimal physiochemcial conditions for the manipulation and short-term preservation of koala (Phascolarctos cinereus) spermatozoa. Journal of Reproduction and Fertility, 118 (2), 273-281. doi: 10.1530/jrf.0.1180273

Holt, W. V., Penfold, L. M., Johnston, S. D., Temple-Smith, P., Shaw, J., McCallum, C., Lindemans, W. and Blyde, D. (1999). Cryopreservation of macropodid spermatozoa: New insights from the cryomicroscope. Reproduction Fertility and Development, 11 (6), 345-353. doi: 10.1071/RD99076

Johnston, S. D., O'Boyle, D., Frost, A. J., McGowan, M. R., Tribe, A. and Higgins, D. (1998). Antibiotics for the preservation of koala (Phascolarctos cinereus) semen. Australian Veterinary Journal, 76 (5), 335-338. doi: 10.1111/j.1751-0813.1998.tb12362.x

Johnston, S. D., Blyde, D., Gamble, J., Higgins, D., Field, H. and Cooper, J. (1997). Collection and short-term preservation of semen from free-ranging eastern grey kangaroos (Macropus giganteus: Macropodidae). Australian Veterinary Journal, 75 (9), 648-651. doi: 10.1111/j.1751-0813.1997.tb15362.x

Johnston, S. D., O'Callaghan, P., McGowan, M. R. and Phillips, N. J. (1997). Characteristics of koala (Phascolarctos cinereus adustus) semen collected by artificial vagina. Journal of Reproduction and Fertility, 109 (2), 319-323. doi: 10.1530/jrf.0.1090319

McGowan, M. R., Tribe, A., Johnston, S. D., Carrick, F. N. and Van Hensbroek, L. (1995). The use of isoflurane to anaesthetise koalas (Phascolarctos cinereus). Australian Veterinary Journal, 72 (12), 472-473. doi: 10.1111/j.1751-0813.1995.tb03495.x

Johnston, S.D., McGowan, M.R., Carrick, F.N. and Cameron, R.D.A. (1994). Seminal characteristics and spermatozoal morphology of captive Queensland koalas, (Phascolarctos cinereus adustus). Theriogenology, 42 (3), 501-511. doi: 10.1016/0093-691X(94)90688-F

Johnston, S. D., McGowan, M. R., Carrick, F. N. and Tribe, A. (1993). Preliminary investigations into the feasibility of freezing koala (Phascolarctos cinereus) semen. Australian Veterinary Journal, 70 (11), 424-425. doi: 10.1111/j.1751-0813.1993.tb06084.x

Johnston, S.D., McGowan, M.R., Carrick, F.N., Tribe, A. and Douglas, R. (1993). Assisted reproductive technology in the koala (Phascolarctos cinereus). Assisted Reproductive Technology, 70, 117-128.

Palacios Benitez, P. D., Duncan, N., Nilesh Haldankar, A., Johnston, S. and Gambini, A. (2023). Koala sperm induces bovine oocyte activation after intracytoplasmic sperm injection. Annual Conference of the International Embryo Technology Society 2024, Denver, CO USA, 9-12 January 2024. Clayton, VIC Australia: CSIRO Publishing. doi: 10.1071/rdv36n2ab35

Johnston, Stephen (2019). Challenges associated with the development and transfer of assisted breeding technology in marsupials and monotremes: lessons from the koala, wombat and short-beaked echidna. Clayton, VIC, Australia: CSIRO Publishing. doi: 10.1071/rd18113

Kollipara, A., Bryan, E., Trimm, L., Carey, A., McLaughlin, E., Johnston, S. and Beagley, K. (2016). Chlamydia infection in males; an underappreciated problem. 13th Congress of the International-Society-for-Immunology-of-Reproduction (ISIR) and the European-Society-for-Reproductive-Immunology (ESRI), Erfurt, Germany, 22-25 June 2016. CLARE: ELSEVIER IRELAND LTD. doi: 10.1016/j.jri.2016.04.160

Dorado, M., Martinez, Gonzalez-M., Gallardo, M., Hebles, M., Sanchez, F., Johnston, S. S. and Gosalvez, J. (2015). The pregnancy and abortion rates of couples with high levels of sperm DNA damage is markedly improved following MACs treatment. 31st Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE), Lisbon, Portugal, 14-17th of June, 2015. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/humrep/30.Supplement_1.1

Fernandez, J. L., Gosalvez, J., Lopez-Fernandez, C., Johnston, S. D. and Esteves, S. C. (2015). Routine determination of sperm DNA fragmentation incorporating the sperm degradation index (SDi) is a useful noninvasive biomarker to identify patients with varicocele in seminograms. 31st Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE), Lisbon, Portugal, 14-17 June 2015. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/humrep/30.Supplement_1.1

Gosalvez, J., Lopez-Fernandez, C., Johnston, S. D., Garci-Ochoa, C., Ruiz-Jorro, M., Martinez-Moya, M., Sanchez-Martin, P., Sanchez-Martin, F., Nunez-Calonge, R. and Caballero, P. (2015). Establishing a threshold level for sperm DNA quality in the semen of donors versus patients using the sperm chromatin dispersion test. 31st Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE), Lisbon, Portugal, 14-17 June 2015. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/humrep/30.Supplement_1.1

Sanchez-Calabuig, M. J., de la Fuente, J., Beltran-Brena, P., Martinez-Nevado, E., Perez-Gutierrez, J. F., Blyde, D., Johnston, S., Harrison, K., Lopez-Fernandez, C. and Gosalvez, J. (2013). Assessment of Fresh and Frozen-Thawed Bottlenose Dolphin (Tursiops Truncatus) Sperm Dna Fragmentation Using the Sperm Chromatin Dispersion Test. Annual Conference of the International Embryo Transfer Society, Hannover, Germany, 19-20 January 2013. Collingwood, VIC., Australia: C S I R O Publishing. doi: 10.1071/RDv25n1Ab170

Ellis, William, Johnston, Steve, Melzer, Alistair, Carrick, Frank, Pyne, Michael and Fitzgibbon, Sean (2013). Disease and ecology in Central Queensland. Conserving Central Queensland's Koalas Workshop, Rockhampton, QLD, Australia, 1–3 February 2012. Rockhampton, QLD, Australia: Koala Research Centre of Central Queensland.

Hogan, LA, Phillips CJC., Janssen T,, Horsup, A, Nicolson V, Keeley T,, Lisle A, and Johnston.SD, (2011). Regular handling reduces behavioural but not physiological stress responses of wombats (Lasiorhinus latifrons) towards humans. Animals and the Environment, a shared responsibility, Brisbane Australia, July, 2011.

Descovich, K, Phillips, C., Nicholson, V., Johnston, S and Lisle, A., (2011). The response of southern hairy-nosed wombats (Lasiorhinus Latifrons) to the presence of faeces. International Ethological Conference and the Animal Behaviour Society annual conference, Indiana University,, July, 2011.

Leehong, P., Ward, L.C., Bryden, W.L., Li, X., Al Jassim, R., Murray, P. and Johnston, S.D. (2009). Using dual energy X-ray absorptiometry (DXA) to measure body composition in the short-beaked Echidna (Tachyglossus aculeatus). Nutrition Society of Australia 2009, Newcastle, Australia, 8-11 Dec, 2009.

Rodger, John C., Paris, Damien B.B.P., Czarny, Natasha A., Harris, Merrilee S., Molinia, Frank C., Taggart, David A., Allen, Camryn D. and Johnston, Stephen D. (2009). Artificial insemination in marsupials. 35th Annual Conference of the International Embryo Transfer Society : Post-conference Satellite Symposium: "Implementation of Artificial Insemination in CANDES”, San Diego, California, USA, 7 January 2009. New York, N.Y., United States: Elsevier. doi: 10.1016/j.theriogenology.2008.09.006

Satake, N., Johnston, S. D. and Holt, W. V. (2009). Sperm Subpopulations in the Koala (Phascolarctos Cinereus) Ejaculate. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv21n1Ab231

Johnston, S. D., Gosalvez, J. and Holt, W. V. (2009). Future proofing Australia's mammalian biodiversity using genome resource banking and art: where are we up to?. 42nd Annual Meeting of the Society for the Study of Reproduction, Pittsburgh, PA United States, 18-22 July 2009. Collingwood, VIC Australia: CSIRO. doi: 10.1071/SRB09ABS046

Seddon, J., Lee, K., Ellis, W., Johnston, S., De Villiers, D., Preece, H. and Carrick, F. (2008). Incorporating spatial data into analysis of koala populations in South-East Queensland. Annual Meeting of the Society of Molecular Biology and Evolution 2008, Barcelona, Spain, 5-8 June, 2008. Society of Molecular Biology and Evolution.

Leehong, P., Cowan, G., Ward, L.C., Li, X., Al Jassim, R., Bryden, W.L., Murray, P., Pyne, M. and Johnston, S.D. (2008). Changes in fat distribution and body composition in the Short-Beaked Echidna (Tachyglossus aculaetus): A clue to seasonal reproduction?. Australian Academic of Sciences Boden Research Conference "Beyond the Platypus Genome", Adelaide, Australia, 23-26 November 2008.

Johnston, S. D., Allen, C, Burridge, M, Mulhall, S, Holt, W, Carrick, F., Lundie-Jenkins, G and Curlewis, J. (2008). Successful production of Koala pouch young following AI using electroejaculated and extended-chilled semen. 16th International Congress on Animal Reproduction, Budapest, Hungary, 13-17 July 2008. Berlin, Germany: Blackwell Wissenschafts Verlag. doi: 10.1111/j.1439-0531.2008.01234.x

Lee, K., Seddon, J., Ellis, W., Johnston, S. and Carrick, F. N. (2007). Genetic variation of closed (captive and island) and open (mainland) Koala populations in Queensland. 53rd meeting of the Australian Mammal Society Conference, University of New England, Armidale, NSW, Australia, 2-4 July 2007.

Lee, K., Seddon, J., Ellis, W., Johnston, S., de Villiers, D., Preece, H. and Carrick, Francis N. (2007). Fine-scale genetic variation in declining Koala populations in South-east Queensland. Fenner Conference on the Environment, Canberra, ACT, 2nd - 5th December 2007. Australasian Wildlife Management Society.

van der Straten, Kaye M., Collette, Bruce B., Leung, Luke K. -P. and Johnston, Stephen D. (2006). Sperm morphology of the black marlin (Makaira indica) differs from scombroid sperm. Miami, FL, United States: Rosenstiel School of Marine and Atmospheric Science.

Zee, Y. P., Allen, C. D., Holt, W. V., Lundie-Jenkins, G., Carrick, F. N. and Johnston, S. D. (2006). Assessment of koala sperm mitochondrial function with JC-1. Thirty-Seventh Annual Conference of the Society of Reproductive Biology, Gold Coast, Qld, 20-23 August, 2006. Victoria: Society of Reproductive Biology.

Johnston, S. D., Smith, B., Pyne, M., Stenzel, D. J. and Holt, W. V. (2006). One-sided ejaculation of sperm bundles in the echidna. 37th Annual Conference of the Society of Reproductive Biology, Gold Coast, 20th - 23rd August, 2007. Victoria: Societ of Reproductive Biology.

Allen, C. D., Burridge, M., Mulhall, S., Nicolson, V., Pyne, M., Zee, Y. P., Curlewis, J. D., Lundie-Jenkins, G., Holt, W. V., Carrick, F. N. and Johnston, S. D. (2006). Successful artificial insemination (AI) in the koala using neat and extended semen collected by electroejaculation (EE). 37th Annual Conference of the Society of Reproductive Biology, Gold Coast, QAld, 20-23 August, 2006. Victoria: Society of Reproductive Biology.

Wells, R. M., Scott, P. T., Harrison, D. K., Cameron, R. D., Johnston, S. D. and D'Occhio, M. J. (2005). Testicular growth factor expression after hemicastration in the neonatal boar. Annual Conference of the Society of Reproductive Biology, Perth, 4-7 September, 2005. Australia: CSIRO Publishing.

Atkin, C. L., D'Occhio, M. J., Johnston, S. D. and Harrison, K. (2005). Testosterone secretion in the Australian sea lion. Annual Conference of the Society of Reproductive Biology, Perth, 4-7 September, 2005. Australia: CSIRO Publishing.

Hengstberger, K. J., Sester, D. P., Tutt, D., Holroyd, R. G., Fordyce, G., Johnston, S. D. and D'Occhio, M. J. (2005). Prevalence of sperm chromatin instability amongst bulls in a subtropical environment: A preliminary investigation. Annual Conference of the Society of Reproductive Biology, Perth, 4-7 September, 2005. Australia: CSIRO Publishing.

Pyne, M. J., D'Occhio, M. J. and Johnston, S. D. (2005). Reproductive physiology and the control of fertility with a GnRH agonist implant in the Australian White Ibis. Showcasing Faculty research through our postgraduates, Gatton, Qld, 8th December 2005. Gatton, Qld: NRAVS Faculty.

Johnston, Steve, Carrick, Frank, Lundie-Jenkins, Geoff and Holt, William (2005). The preservation and management of koala genetic diversity using reproductive biotechnology and molecular genetics: a model for endangered marsupials. AWMS Conference 2005: 18th Scientific Meeting and Annual General Meeting, Hobart, Tasmania, Australia, 21 – 24 November, 2005.

Hogan, L. A., Johnston, S. D., Phillips, C. J. C., Horsup,, Nicolson,, Keeley, and Walton, (2005). The captive management of southern hairy-nosed wombats: a model for the future captive husbandry and reproductive management of northern hairy-nosed wombats. Showcasing Faculty research through our postgraduates, Gatton, Qld, 8th December 2005. Gatton, Qld: NRAVS Faculty.

Johnston, S. D. (2005). Spermiogenesis, epididymal Maturation and Cryopreservation of Grey-headed Flying Fox (Pteropus poliocephalus) Spermatozoa. Showcasing Faculty research through our postgraduates, Gatton, Qld, 8th December 2005. Gatton, Qld: NRAVS Faculty.

Larsen, J. E., Norman, S.T. and Johnston, S.D. (2004). Can vaginal electrical impedance be used to characterize oestrus and ovulation in the mare?. Research Conference Proceedings 2004, Gatton, 5th November 2004. Australia:

MacCallum, C. A., Johnston, S. D. and Blyde, D. (2003). Lack of seasonal variation in male reproductive parameters measured in a captive and wild population of common wombats in New South Wales. Society for Reproductive Biology Thirty-fourth Annual Conferen, Melbourne, Victoria, 14-17 September 2003. Australia: CSIRO.

Johnston, S. D., O'Callaghan, P, Nilsson, K, Tzipori, G., Strong, H. and Curlewis, J. D. (2003). Semen-induced luteal phase in the koala. Society for Reproductive Biology Thirty-fourth Annual Conference, Melbourne, Victoria, 14-17 September 2003. Australia: CSIRO.

McClean, R. V. and Johnston, S. D. (2003). Cryopathology of macropod spermatozoa. Society for Reproductive Biology Thirty-fourth Annual Conference, Melbourne, Victora, 14-17 September 2003. Australia: CSIRO.

MacCallum, C. A., Johnston, S. D. and Blyde, D. (2002). A comparative investigation into the osmotic tolerance of wombat and koala spermatozoa. Proceedings of the 28th Annual Conference of the International Embryo Transfer Society, Foz do Iguacu, Brazil, 12 January - 15 January. New York: Elsevier Science Inc.

Johnston, S. D., O'Callaghan, P. O. and McGowan, M. (2001). Development of an artificial insemination prorocol in the koala (Phascolarctos cinerus). Zoo & Exotic Diagnostics: British Veterinary Zoological Society Autumn Meeting, Royal Veterinary College, Potters Bar, 10-11 November, 2001.

Johnston, S. D., O'Callaghan, P., Nilsson, K. and Curlewis, J. D. (2001). Post-coital LH surge in the koala (Phascolarctos cinereus). Society for Reproductive Biology - 32nd Annual Conference, Gold Coast, QLD, 9th - 12th September, 2001. Victoria: Society for Reproductive Biology.

Johnston, S. D. and Holt, W. V. (2001). Assisted breeding technology in the Marsupialia: Using the koala as a reproductive model.. Proceedings of the First International Symposium on Assisted Reproductive Technology for the Conservation and Genetic Management of Wildlife, Henry Dooly Zoo, Omaha, Nebraska, 17-18 January 2001. Omaha, Nebraska, USA: Henry Doorly Zoo.

MacCallum, C. A., Johnston, S. D., Ward, D. and Blyde, D. (2001). Successful cryopreservation of common wombat (Vombatus ursinus) spermatozoa recovered from refrigerated epididymidal tissue. Proceedings of the Annual Conference of the International Embryo Transfer Society, Henry Doorly Zoo, Nebraska, USA, January 2001. New York: Elsevier Science Inc.

Phillips, N., Mcgowan, M. R., Morton, J., Mayer, D. G., Johnston, S. D. and Rodrig, H. (2000). The relationship between conception rate and measures of frozen-thawed sperm viability for eleven high-use Australian A.I. dairy bulls. International Congress on Animal Reproduction, Stockholm, Sweden, 2nd - 6th July, 2000. Stockholm, Sweden: Repro Print AB.

Holt, W.V. and Johnston, S.D. (1999). Enhanced osmotic tolerance of Koala spermatozoa following treatment with the cytoskeletal disrupting agent, cytochalasin D. 30th Anual Conference of Australian Society for Reproductive Biology, Melbourne, Vic, Australia, 26-29 September, 1999. Melbourne, VIC, Australia: Australian Society for Reproductive Biology.

Johnston, S.D., McGowan, M.R. and O'Callaghan, P. (1999). Assisted breeding technology for the conservation and propagation of Phascolarctos cinereus or How to make a koala pouch young. Postgraduate Committee in Veterinary Science, Sydney, University of Sydney, 1999.

Johnston, S. D., McGowan, M. R., O'Callaghan, P., Cox, R., Houlden, B., Haig, S. and Taddeo, G. (1999). Birth of koala pouch young following artificial insemination. Australian Society of Reproductive Biologists, Melbourne, -.

Johnston, S.D., McGowan, M.R., O'Callaghan, P., Cox, R. and Nicolson, V. (1997). Evidence for reflex ovulation in the koala. 28th Annual Conference Australian Society of Reproductive Biology, Canberra, ACT, Australia, 29 September - 1 October, 1997.

Johnston, S.D., O'Callaghan, P. and McGowan, M.R. (1996). Seminal characteristics of koala ejaculates collected using an artificial vagina. Thirteenth International Conference of Animal Reproduction, Rockhampton, Qld, Australia, 26-28 June, 1996.

Johnston, S.D., Blyde, D., Gamble, J., Higgins, D., Field, H. and Cooper, J. (1996). Developing field methods for the collection and preservation of semen from endangered species of kangaroos. Thirteenth International Conference of Animal Reproduction, Rockhampton, Qld, Australia, 26-28 June, 1996.

Cooper, J.W., Johnston, S.D., Blyde, D., Kuiper, Y., McGowan, M.R. and Booth, R. (1996). Effect of glycerol and egg yolk on the motility of Macropus giganteus cauda epididymidal spermatozoa stored at 5oC. Thirteenth International Conference of Animal Reproduction, Rockhampton, Qld, Australia, 26-28 June, 1996.

Johnston, S.D., Daddow, L. and Carrick, F.N. (1994). Observations of mature spermatozoa from the Greater Bilby (Macrotis lagotis). Seventh International Spermatology Symposium, Cairns, Qld, Australia, 9-14 October, 1994.

Tribe, A., Johnston, S.D. and Carrick, F.N. (1994). Artificial reproduction in marsupials. In Australian Wildlife. 110th Postgraduate Committee in Veterinary Science, Sydney, University of Sydney, 1994. Sydney, NSW, Australia: WildLife Preservation Society of Australia.

Johnston, S.D., McGowan, M.R., Carrick, F.N. and Cameron, R.D.A. (1992). Towards the development of an artificial insemination programme for koalas (Phascolarctos cinereus). 12th International Congress on Animal Reproduction, Haig, The Netherlands, 23-27 August, 1992.

Lees, Angela, Gaughan, John, Wallage, Andrea and Johnston, Steve (2024). Complete multimodel dataset. The University of Queensland. (Dataset) doi: 10.48610/fb62e47

Johnston, Stephen Douglas (1999). Studies towards the development of an artificial insemination protocol in the koala, Phascolarctos cinereus. PhD Thesis, School of Veterinary Science and Animal Production, The University of Queensland. doi: 10.14264/uql.2017.511

Johnston, Stephen, Lever, John, McLeod, Robby, Oishi, Motoharu and Collins, Simon (2014). Development of breeding techniques in the crocodile industry. Canberra, ACT, Australia:

Phillips, N., McGowan, M. R., Johnston, S. D., Mayer, D., Morton, J. and Carrick, M (2001). Relationship between post-thaw sperm characteristics and the corrected conception rate (CCR) of selected high use Australian dairy AI sires. Melbourne: Dairy Research & Development Corp.

Associate Professor Steve Johnston

PhD and MPhil Supervision

Publications

Book Chapters

Journal Articles

Conference Papers

Data Collection

Thesis

Research Reports